Structure
(Structure of B1750-51)
Biotin Protein
Biotin (Long Arm) Maleimide
Applications
ELISA
Synonyms
Biotin; Biotin (Long Arm) Maleimide; Biotin protein
Applicable Applications for Biotin protein
Suitable for use in ELISA. Other applications not tested.
Application Notes
Optimal dilutions to be determined by the researcher.
Molecular Formula
C22H33N5O5S
Target for Labeling
DNA, Oligonucleotides, PNA, Protein, RNA
Required Reactive Group
Thiol Group
Tag/Group Incorporated
Biotin
Protein Labeling Procedure
1. Dissolve the protein to be labeled in 100mM PBS, pH 7.0, to a concentration of 5mg/ml.
2. Dissolve a slight excess of MBS635054 in DMF at a concentraton of 20mg/ml.
3. Add 25ul/ml of MBS635054 of protein solution.
4. Incubate at RT for 3hrs with occasional stirring.
5. Separate the unreacted material from the protein by gel filtration or dialysis.
2. Dissolve a slight excess of MBS635054 in DMF at a concentraton of 20mg/ml.
3. Add 25ul/ml of MBS635054 of protein solution.
4. Incubate at RT for 3hrs with occasional stirring.
5. Separate the unreacted material from the protein by gel filtration or dialysis.
Labeled DNA Control
(Included)
(Included)
Supplied as a liquid in 10mM Tris, pH 8.0, 1mM EDTA of, 50ul of Hind III.
This standard is supplied as a reference standard in FastTag nucleic acid labeling protocols to verify successful labeling.
This standard is supplied as a reference standard in FastTag nucleic acid labeling protocols to verify successful labeling.
Reconstitution
FastTag labeling: Reconstitute with 500ul anhydrous DMF.
NOTE: It is not recommended to store the solution.
5' EndTag or 3'EndTag labeling: Reconstitute with 312ul anhydrous DMF solution
NOTE: It is not recommended to store the solution.
Follow the labeling procedure included with the 5' EndTag or 3' EndTag systems.
NOTE: It is not recommended to store the solution.
5' EndTag or 3'EndTag labeling: Reconstitute with 312ul anhydrous DMF solution
NOTE: It is not recommended to store the solution.
Follow the labeling procedure included with the 5' EndTag or 3' EndTag systems.
Preparation and Storage
Lyophilized product is stable for 6 months after receipt at -20°C.
Reconstitute with sterile DMF. It is not recommended to store reconstituted solution.
Reconstitute with sterile DMF. It is not recommended to store reconstituted solution.
Structure
(Structure of B1750-51)
Structure
(Structure of B1750-51)
Product Categories/Family for Biotin protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
biotin
Protein Family
Similar Products
Product Notes
The Biotin (Catalog #AAA635054) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Biotin can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ELISA. Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Biotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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