Agouti Protein | ASIP protein

Agouti (Control Peptide)

Cat. Num. AAA634855

• Gene Names: ASIP; ASP; AGSW; AGTI; AGTIL; SHEP9
• Applications: ELISA
• Synonyms: Agouti; Agouti (Control Peptide); ASIP protein

• Specificity: A 21 amino acid control peptide for MBS618407 near the N-terminus of mouse Agouti (1). The sequence shows no significant sequence homology with AGRP, tubby, tub, TULP1 or TULP2. Species Sequence Homology: rat; 90%, human and monkey; 80%, porcine; 73%, fox; 75%, bovine; 66%
• Form/Format: Supplied as a lyophilized powder from PBS, pH7.5. Reconstitute with 100ul sterile PBS.
• Concentration: ~1mg/ml(after reconstitution) (varies by lot)

• Applicable Applications for ASIP protein: ELISA (EL/EIA), Antibody blocking experiments
• Application Notes: ELISA: 1:10,000-1:100,000 using 50-100ng/well; Antibody Blocking: 5-10ug control peptide/1ug of MBS618407 or 1ul of MBS613352.; ; Suitable for use in ELISA and antibody blocking experiments. Not recommended for use in Western Blot. Other applications not tested. Optimal dilutions to be determined by the researcher.
• Preparation and Storage: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ASIP protein

Agouo protein (132 aa in human) is normally expressed in skin but its ubiquitous expression causes obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melonocortin receptor 1 (MVC-1). However, recombinant agouti protien also antagonizes the MC2R and MC4R. ARGP (132aa in human, chromosomes 16q21) is normally expressed in adrenal and hypothalamus. AGRP levels are increased several folds in ob/ob mice. AGRP is a strong antagonist of MC3R ans MV4R. Ubiquitous expression of AGRP in transgenic mice causes obesity without altering skin pigmentation.

Product Categories/Family for ASIP protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 540073
• NCBI GeneID: 434
• UniProt Accession #: P42127; Q3SXL2
• NCBI Official Full Name: agouti
• NCBI Official Synonym Full Names: agouti signaling protein
• NCBI Official Symbol: ASIP
• NCBI Official Synonym Symbols: ASP; AGSW; AGTI; AGTIL; SHEP9
• NCBI Protein Information: agouti-signaling protein; nonagouti homolog; agouti switch protein; agouti signaling protein, nonagouti homolog
• UniProt Protein Name: Agouti-signaling protein
• Protein Family: Agouti-related protein
• UniProt Gene Name: ASIP
• UniProt Synonym Gene Names: AGTI; AGTIL; ASP; ASP
• UniProt Entry Name: ASIP_HUMAN

NCBI Description

In mice, the agouti gene encodes a paracrine signaling molecule that causes hair follicle melanocytes to synthesize pheomelanin, a yellow pigment, instead of the black or brown pigment, eumelanin. Pleiotropic effects of constitutive expression of the mouse gene include adult-onset obesity, increased tumor susceptibility, and premature infertility. This gene is highly similar to the mouse gene and encodes a secreted protein that may (1) affect the quality of hair pigmentation, (2) act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone, (3) play a role in neuroendocrine aspects of melanocortin action, and (4) have a functional role in regulating lipid metabolism in adipocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

ASIP: Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). In higher primates, agouti may affect the quality of hair pigmentation rather than its pattern of deposition. Could well play a role in neuroendocrine aspects of melanocortin action. May have some functional role in regulating the lipid metabolism with adipocytes.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q11.2-q12

Cellular Component: extracellular space

Molecular Function: type 3 melanocortin receptor binding; type 4 melanocortin receptor binding; receptor binding

Biological Process: melanin biosynthetic process; generation of precursor metabolites and energy; regulation of molecular function, epigenetic; hormone-mediated signaling; cell-cell signaling; melanosome organization and biogenesis; adult feeding behavior; signal transduction; melanosome transport

Disease: Skin/hair/eye Pigmentation, Variation In, 9