PROK2 recombinant protein

PROK2

Cat. Num. AAA553703

• Gene Names: PROK2; BV8; HH4; PK2; KAL4; MIT1
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: PROK2; PROK2 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: AVITGACDKD SQCGGGMCCA VSIWVKSIRI CTPMGKLGDS CHPLTRKVPF FGRRMHHTCP CLPGLACLRT SFNRFICLAQ K
• Sequence Length: 129

• Applicable Applications for PROK2 recombinant protein: SDS-PAGE
• Application Notes: Recombinant PROK2 migrates as an approximately 9 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human PROK2 mature chain was expressed in ECOLI. Recombinant Human Prokineticin-2 encodes amino acid residues 28-129.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 187167261
• NCBI GeneID: 60675
• NCBI Accession #: NP_001119600.1
• NCBI GenBank Nucleotide #: NM_001126128.2
• UniProt Accession #: Q9HC23; Q53Z79; Q6ISR0
• Molecular Weight: 11,659 Da
• NCBI Official Full Name: prokineticin-2 isoform a
• NCBI Official Synonym Full Names: prokineticin 2
• NCBI Official Symbol: PROK2
• NCBI Official Synonym Symbols: BV8; HH4; PK2; KAL4; MIT1
• NCBI Protein Information: prokineticin-2
• UniProt Protein Name: Prokineticin-2
• Protein Family: Prokineticin
• UniProt Gene Name: PROK2
• UniProt Synonym Gene Names: BV8; PK2

NCBI Description

This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle.