Rabbit anti-Mouse p21Cip1 Polyclonal Antibody | anti-p21Cip1 antibody

p21Cip1, Phosphorylated (S78) (Cdkn1a, Cip1, Waf1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21) (Azide free) (HRP)

Cat. Num. AAA6330113

• Reactivity: Mouse
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: p21Cip1; Polyclonal Antibody; Phosphorylated (S78) (Cdkn1a; Cip1; Waf1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein; p21) (Azide free) (HRP); Cdkn1a; p21; anti-p21Cip1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-p21Cip1 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse p21Cip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S78 of mouse p21Cip1.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Mouse p21Cip1 Antibody (Phospho S78) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-p21Cip1 antibody

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-p21Cip1 antibody

Antibodies; Protein Kinase Inhibitors

References

Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Kim, J., et al. Cytokine 50(1):42-49(2010). Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010). Gumina, M.R., et al. Cell Cycle 9(4):820-828(2010). Radulovich, N., et al. Mol. Cancer 9, 24 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 209862835
• NCBI GeneID: 896
• NCBI Accession #: NP_001129489.1
• NCBI GenBank Nucleotide #: NM_001136017.2
• UniProt Accession #: P39689; Q5T8J0; Q6FG62; Q96F49; B2RD63; B3KQ22; E9PAS4; E9PB36; P30281
• Molecular Weight: 32,520 Da
• NCBI Official Full Name: G1/S-specific cyclin-D3 isoform 1
• NCBI Official Synonym Full Names: cyclin D3
• NCBI Official Symbol: CCND3
• NCBI Protein Information: G1/S-specific cyclin-D3; D3-type cyclin; G1/S-specific cyclin D3
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: Cdkn1a
• UniProt Synonym Gene Names: Cip1; Waf1
• UniProt Entry Name: CDN1A_MOUSE

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]