Rabbit anti-Human PRKAR1A Polyclonal Antibody

PRKAR1A, NT (cAMP-dependent Protein Kinase Type I-alpha Regulatory Subunit, Tissue-specific Extinguisher 1, TSE1, PRKAR1, PKR1)

Cat. Num. AAA625570

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PRKAR1A; Polyclonal Antibody; NT (cAMP-dependent Protein Kinase Type I-alpha Regulatory Subunit; Tissue-specific Extinguisher 1; TSE1; PRKAR1; PKR1); Anti -PRKAR1A; anti-PRKAR1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKR1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PRKAR1A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human PKR1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PKR1 Antibody (N-term) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PKR1 (arrow) using rabbit polyclonal hPKR1-M1 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKR1 gene (Lane 2).(2ug/ml))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PRKAR1A antibody

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKR1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in the gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. The gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.

Product Categories/Family for anti-PRKAR1A antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 150022369
• NCBI Official Full Name: protein kinase A regulatory subunit type I/alpha-retinoic acid receptor alpha fusion protein isoform b
• Protein Family: cAMP-dependent protein kinase