Rabbit KRAS Polyclonal Antibody | anti-KRAS antibody

KRAS, CT (KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed) (PE)

Cat. Num. AAA6314457

• Gene Names: KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KRAS; Polyclonal Antibody; CT (KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; N-terminally processed) (PE); N-terminally processed; anti-KRAS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRAS. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KRAS antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 146-174 near the C-terminal region of human KRAS.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HT-29 whole cell lysate (20ug/lane) using MBS645347 (1:2000). Secondary: IgG (HRP) Goat Anti-Rabbit at 1:10000 dilution. Predicted band size : 22kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot using MBS645347 at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: C2C12 whole cell lysate Lane 4: C6 whole cell lysate Lane 5: HT-29 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20ug per lane. Secondary antibocy: IgG (HRP) Goat Anti-Rabbit, at 1:10000 Predicted band size : 22kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot using MBS645347 at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: K562 whole cell lysate Lane 5: PC-12 whole cell lysate Lane 5: Ramos whole cell lysate Lysates/proteins at 20ug per lane. Secondary antibody: IgG (HRP) Goat Anti-Rabbit 1:10000 dilution. Predicted band size : 22kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of WiDr cells using MBS645347 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-KRAS antibody

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Product Categories/Family for anti-KRAS antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010). 2. Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010). 3. Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010). 4. Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010). 5. Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 15718763
• NCBI GeneID: 3845
• NCBI Accession #: NP_203524.1; NP_004976.2
• NCBI GenBank Nucleotide #: NM_033360.3
• UniProt Accession #: P01116; P01118; Q96D10; A8K8Z5; B0LPF9
• Molecular Weight: 21,425 Da
• NCBI Official Full Name: GTPase KRas isoform a
• NCBI Official Synonym Full Names: Kirsten rat sarcoma viral oncogene homolog
• NCBI Official Symbol: KRAS
• NCBI Official Synonym Symbols: NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• NCBI Protein Information: GTPase KRas; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
• UniProt Protein Name: GTPase KRas
• Protein Family: GTPase
• UniProt Gene Name: KRAS
• UniProt Synonym Gene Names: KRAS2; RASK2
• UniProt Entry Name: RASK_HUMAN

Uniprot Description

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; G protein; G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2