SDS-Page
(Recombinant PRKACG protein gel 10% SDS-PAGE Coomassie staining MW: 41.7 kDa Purity: ?80%)
PRKACG recombinant protein
Recombinant PRKACG protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PRKACG protein gel 10% SDS-PAGE Coomassie staining MW: 41.7 kDa Purity: ?80%)
SDS-Page
(Recombinant PRKACG protein gel 10% SDS-PAGE Coomassie staining MW: 41.7 kDa Purity: ?80%)
Testing Data
(HTRF assay for recombinant PRKACG protein activity 1 uM STK S3 substrate was incubated with different concentrations of PRKACG protein in a reaction buffer containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 ?M ATP for 1 hr. Detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer ,were added and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for recombinant PRKACG protein activity 1 uM STK S3 substrate was incubated with different concentrations of PRKACG protein in a reaction buffer containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 ?M ATP for 1 hr. Detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer ,were added and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for recombinant PRKACG protein activity 1 uM STK S3 substrate was incubated with different concentrations of PRKACG protein in a reaction buffer containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 ?M ATP for 1 hr. Detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer ,were added and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for recombinant PRKACG protein activity 1 uM STK S3 substrate was incubated with different concentrations of PRKACG protein in a reaction buffer containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 ?M ATP for 1 hr. Detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer ,were added and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
Background: PRKACG (Protein Kinase CAMP-Activated Catalytic Subunit Gamma), also called as PKACG or KAPG, is a member of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatorysubunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. PRKACG can phosphorylate a large number of substrates in the cytoplasm and the nucleus. PRKACG has been shown to interact with Ryanodine receptor 2. Diseases associated with PRKACG include Bleeding Disorder, Platelet-Type, 19 and Stormorken Syndrome. Among its related pathways are Melanocyte Development and Pigmentation and Ovarian steroidogenesis.
NCBI and Uniprot Product Information
NCBI Description
Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
PKACG: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript. Activated by cAMP. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Protein type: Kinase, protein; Protein kinase, AGC; EC 2.7.11.11; Protein kinase, Ser/Thr (non-receptor); AGC group; PKA family
Chromosomal Location of Human Ortholog: 9q13
Cellular Component: cytosol
Molecular Function: cAMP-dependent protein kinase activity; ATP binding
Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; male gonad development; glucose metabolic process; pathogenesis; signal transduction; protein amino acid phosphorylation; gluconeogenesis; phospholipase C activation; carbohydrate metabolic process; triacylglycerol catabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; spermatogenesis; blood coagulation; transmembrane transport; regulation of insulin secretion
Disease: Mental Retardation, Autosomal Dominant 31
Research Articles on PRKACG
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Product Notes
The PRKACG prkacg (Catalog #AAA389235) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRKACG, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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