Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit | PRKAA1 elisa kit

Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit

Cat. Num. AAA764788

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human
• Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1; Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit; PRKAA1/2.7.11.26/Tau-protein kinase PRKAA1)/2.7.11.27/Tau-protein kinase PRKAA1 (ACACA kinase)/2.7.11.31/Hydroxymethylglutaryl-CoA reductase kinase (HMGCR kinase)/AMPK1/AMPK subunit alpha-1; PRKAA1 elisa kit

• Reactivity: Human
• Sequence Length: 559

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PRKAA1 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRKAA1. During the reaction,Human PRKAA1 in the sample or standard competes with a fixed amount of biotin-labeled Human PRKAA1 for sites on a pre-coated Monoclonal antibody specific to Human PRKAA1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human PRKAA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94557301
• NCBI GeneID: 5562
• NCBI Accession #: NP_006242.5
• NCBI GenBank Nucleotide #: NM_006251.5
• Molecular Weight: 65,523 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
• NCBI Official Synonym Full Names: protein kinase AMP-activated catalytic subunit alpha 1
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.26; Autophagy; Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; EC 2.7.11.27; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.31; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: AMP-activated protein kinase complex; apical plasma membrane; cytoplasm; cytosol; intracellular; nucleus

Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; chromatin binding; histone serine kinase activity; kinase binding; metal ion binding; protein binding; protein C-terminus binding; protein kinase activity; tau-protein kinase activity

Biological Process: activation of MAPK activity; cell cycle arrest; cellular response to glucose starvation; cellular response to nutrient levels; cholesterol biosynthetic process; cold acclimation; fatty acid biosynthetic process; fatty acid homeostasis; fatty acid oxidation; gene expression; glucose homeostasis; glucose metabolic process; insulin receptor signaling pathway; lipid biosynthetic process; macroautophagy; negative regulation of apoptosis; negative regulation of glucosylceramide biosynthetic process; negative regulation of lipid catabolic process; negative regulation of TOR signaling pathway; positive regulation of autophagy; positive regulation of cell proliferation; positive regulation of cholesterol biosynthetic process; positive regulation of glycolysis; positive regulation of skeletal muscle development; protein amino acid phosphorylation; protein heterooligomerization; regulation of circadian rhythm; regulation of peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; response to activity; response to caffeine; response to gamma radiation; response to hypoxia; response to UV; rhythmic process; signal transduction; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway