CLPS/Colipase Active Protein | CLPS active protein

Recombinant Human CLPS/Colipase Protein

Cat. Num. AAA9139722

• Purity: >90% by SDS-PAGE.
• Synonyms: CLPS/Colipase; Recombinant Human CLPS/Colipase Protein; CLPS active protein

• Host: HEK293 Cells
• Purity/Purification: >90% by SDS-PAGE.
• Form/Format: Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
• Sequence: MEKILILLLVALSVAYAAPGPRGIIINLENGELCMNSAQCKSNCCQHSSALGLARCTSMASENSECSVKTLYGIYYKCPCERGLTCEGDKTIVGSITNTNFGICHDAGRSKQ
• Sequence Length: 112

• Species: Human
• Endotoxin: < 0.1 EU/ug of the protein by LAL method.
• Biological Activity: Measured by its ability to block I-309-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR8. The ED50 for this effect is 15-75 ng/mL in the presence of 20 ng/mL rhI-309.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
• Tag: 6xHis tag at the C-terminus
• Preparation and Storage: Store the lyophilized protein at -20 degree C to -80 degree C for long term.; After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.

SDS-Page

(Recombinant Human CLPS/Colipase Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 13 kDa.)

Related Product Information for CLPS active protein

Description: Recombinant Human CLPS/Colipase Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Gln112) of human CLPS/Colipase (Accession #NP_001823.1) fused with a 6xHis tag at the C-terminus.

Background: The protein is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for CLPS active protein

Other Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4502895
• NCBI GeneID: 1208
• NCBI Accession #: NP_001823
• NCBI GenBank Nucleotide #: NP_001823.1
• UniProt Accession #: P04118
• NCBI Official Full Name: colipase isoform 1 preproprotein
• NCBI Official Synonym Full Names: colipase
• NCBI Official Symbol: CLPS
• NCBI Protein Information: colipase
• UniProt Protein Name: Colipase
• Protein Family: Colipase
• UniProt Gene Name: CLPS

NCBI Description

The protein encoded by this gene is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilizing an active conformation and considerably increasing the overall hydrophobic binding site. The gene product allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. This cofactor is only expressed in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

Colipase is a cofactor of pancreatic lipase. It allows the lipase to anchor itself to the lipid-water interface. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase.