Typical Testing Data/Standard Curve (for reference only)
Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein ELISA Kit | PREX1 elisa kit
Human Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Rex1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for P-Rex1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain P-Rex1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of P-Rex1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
PREX1: Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. Interacts preferentially with RAC2. Interacts with RAC1. Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: GEF; GEF, Rac/Rho
Chromosomal Location of Human Ortholog: 20q13.13
Cellular Component: cytosol; plasma membrane
Molecular Function: enzyme binding; GTPase activator activity; guanyl-nucleotide exchange factor activity; phospholipid binding; protein binding; Rac guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity
Biological Process: actin filament polymerization; G-protein coupled receptor protein signaling pathway; negative regulation of protein kinase activity; negative regulation of TOR signaling pathway; neutrophil activation; positive regulation of apoptosis; regulation of small GTPase mediated signal transduction; superoxide metabolic process
Research Articles on PREX1
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Product Notes
The Human PREX1 prex1 (Catalog #AAA9428400) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9428400 ELISA Kit recognizes Human PREX1. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol 3, 4, 5-trisphosphate-dependent Rac exchanger 1 protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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