Protein Phosphatase 2 Recombinant Protein | PPP2R1A recombinant protein

Recombinant Human Protein Phosphatase 2, Regulatory Subunit A Alpha

Cat. Num. AAA141161

• Gene Names: PPP2R1A; MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Protein Phosphatase 2; Recombinant Human Protein Phosphatase 2; Regulatory Subunit A Alpha; PPP2R1A Human; Regulatory Subunit A Alpha Human Recombinant; PPP2R1A; Regulatory Subunit A; Alpha; Protein Phosphatase 2 (Formerly 2A); Regulatory Subunit A (PR 65); Alpha Isoform; PP2A Subunit A Isoform PR65-Alpha; PP2A Subunit A Isoform R1-Alpha; Serine/Threonine Protein Phosphatase 2A; 65 KDa Regulatory Subunit A; Serine/Threonine-Protein Phosphatase 2A; 65 KDa Regulatory Subunit A Alpha Isoform; Medium Tumor Antigen-Associated 61 KDA Protein; Medium Tumor Antigen-Associated 61 KDa Protein; 65kDa Regulatory Subunit A; Protein Phosphatase 2A; PP2A-Aalpha; PP2AAALPHA; PR65A; PPP2R1A recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: PPP2R1A protein solution (1mg/ml) containing Phosphate Buffered Saline (pH7.4) and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAADGD DSLYPIAVLI DELRNEDVQL RLNSIKKLST IALALGVERT RSELLPFLTD TIYDEDEVLL ALAEQLGTFT TLVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR VSSAVKAELR QYFRNLCSDD TPMVRRAAAS KLGEFAKVLE LDNVKSEIIP MFSNLASDEQ DSVRLLAVEA CVNIAQLLPQ EDLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDANQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA
• Sequence Length: 589

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for PPP2R1A recombinant protein

Introduction: Protein Phosphatase 2, Regulatory Subunit A Alpha, also known as PPP2R1A is part of the PPP family of phosphatases, which regulates a diversity of vital cellular processes. PPP2R1A subunit, PR65, functions as a scaffolding molecule which coordinates the assembly of the catalytic subunit and a variable regulatory B subunit. PPP2R1A is essential for proper chromosome segregation as well as for centromeric localization of SGOL1 in mitosis. Furthermore, PPP2R1A phosphorylation is an in vivo mechanism which regulates the PP2A signaling complex as well as increasing the PP2A activity in heart failure.

Description: PPP2R1A Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 612 amino acids (1-589 a.a) and having a molecular mass of 67.7kDa.PPP2R1A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Categories/Family for PPP2R1A recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 21361399
• NCBI GeneID: 5518
• NCBI Accession #: NP_055040.2
• NCBI GenBank Nucleotide #: NM_014225.5
• UniProt Accession #: P30153; Q13773; Q6ICQ3; Q96DH3
• Molecular Weight: 65,309 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit A, alpha
• NCBI Official Symbol: PPP2R1A
• NCBI Official Synonym Symbols: MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPP2R1A
• UniProt Entry Name: 2AAA_HUMAN

Uniprot Description

PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: chromosome, pericentric region; cytosol; protein phosphatase type 2A complex

Molecular Function: antigen binding; protein binding; protein heterodimerization activity; protein phosphatase regulator activity

Biological Process: apoptosis; chromosome segregation; G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation; protein complex assembly

Disease: Mental Retardation, Autosomal Dominant 36