Rabbit anti-Human PPP2R1A Polyclonal Antibody | anti-PPP2R1A antibody

Anti-PPP2R1A Antibody, Rabbit Polyclonal

Cat. Num. AAA8104011

• Gene Names: PPP2R1A; MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PPP2R1A; Polyclonal Antibody; Anti-PPP2R1A Antibody; Rabbit Polyclonal; PPP2R1A Antibody; Rabbit PAb; Antigen Affinity Purified; protein phosphatase 2; regulatory subunit A; alpha; Anti-PP2A-Aalpha Antibody; Anti-PP2AAALPHA Antibody; Anti-PR65A Antibody; anti-PPP2R1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PPP2R1A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PPP2R1A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human PPP2R1A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PPP2R1A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PPP2R1A rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:65 kDa Observed band size:65 kDa)

Related Product Information for anti-PPP2R1A antibody

The PPP2R1A gene on chromosome 19 (19q13.41) encodes the alpha isoform of the scaffolding subunit of the PP2A holoenzyme, which functions to link the catalytic subunit to the regulatory subunit. Somatic missense mutations in the Ser/Thr protein phosphatase 2A (PP2A) Aalpha scaffold subunit gene PPP2R1A are among the few genomic alterations that occur frequently in serous endometrial carcinoma (EC) and carcinosarcoma, two clinically aggressive subtypes of uterine cancer with few therapeutic options. The PPP2R1A mutation regions are conserved among species and known to interact with the regulatory subunits of the PP2A enzyme. Subtype-specific PPP2R1A mutations have been frequently observed in ovarian and endometrial cancer. PPP2R1A mutant endometrial cancers may represent good candidates for personalized drug therapies particularly for women with the lethal serous histologic variant of uterine cancer.

References

Wang F, et al. (2013) Infrequent mutations of the ppp2r1a and ppp2r1b genes in patients with ovarian cancer. Mol Med Rep 7 (6): 1826-1830.Wallace A, et al. (2019) A newborn with severe ventriculomegaly: Expanding the ppp2r1a gene mutation phenotype. J Pediatr Genet 8 (4): 240-243.Nagendra DC, et al. (2012) Ppp2r1a mutations are common in the serous type of endometrial cancer. Mol Carcinog 51 (10): 826-831.Haesen D, et al. (2016) Recurrent ppp2r1a mutations in uterine cancer act through a dominant-negative mechanism to promote malignant cell growth. Cancer Res 76 (19): 5719-5731.

NCBI and Uniprot Product Information

• NCBI GI #: 21361399
• NCBI GeneID: 5518
• NCBI Accession #: NP_055040.2
• NCBI GenBank Nucleotide #: NM_014225.5
• UniProt Accession #: P30153; Q13773; Q6ICQ3; Q96DH3
• Molecular Weight: 65,309 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit A, alpha
• NCBI Official Symbol: PPP2R1A
• NCBI Official Synonym Symbols: MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Gene Name: PPP2R1A
• UniProt Entry Name: 2AAA_HUMAN

Uniprot Description

PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: chromosome, pericentric region; cytosol; protein phosphatase type 2A complex

Molecular Function: antigen binding; protein binding; protein heterodimerization activity; protein phosphatase regulator activity

Biological Process: apoptosis; chromosome segregation; G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation; protein complex assembly

Disease: Mental Retardation, Autosomal Dominant 36