SDS-Page
PPP2R1A recombinant protein
PPP2R1A, 1-589aa, Human, His tag, E Coli
Gene Names
PPP2R1A; MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
PPP2R1A; 1-589aa; Human; His tag; E Coli; Serine/Threonine-Protein Phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A-Aalpha; PP2AAALPHA; PR65A; PPP2R1A recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAADGD DSLYPIAVLI DELRNEDVQL RLNSIKKLST IALALGVERT RSELLPFLTD TIYDEDEVLL ALAEQLGTFT TLVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR VSSAVKAELR QYFRNLCSDD TPMVRRAAAS KLGEFAKVLE LDNVKSEIIP MFSNLASDEQ DSVRLLAVEA CVNIAQLLPQ EDLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDA NQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA
Sequence Length
589
Applicable Applications for PPP2R1A recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PPP2R1A recombinant protein
PPP2R1A also known as Serine/Threonine-Protein Phosphatase 2A 65 kDa regulatory subunit A alpha isoform, is a member of the PPP family of phosphatases, regulates a variety of essential cellular processes. PPP2R1A phosphorylation is an in vivo mechanism for regulation of the PP2A signaling complex and increased PP2A activity in heart failure. Recombinant human PPP2R1A, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PPP2R1A recombinant protein
References
Yang R., et al. (2013) PLoS One. 8 (10):e77285.; Li H H., et al. (2007) EMBO J. 26 (2): 902-914.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67.7kDa (612aa)
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
NCBI Official Synonym Full Names
protein phosphatase 2 scaffold subunit Aalpha
NCBI Official Symbol
PPP2R1A
NCBI Official Synonym Symbols
MRD36; PP2AA; PR65A; PP2AAALPHA; PP2A-Aalpha
NCBI Protein Information
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Gene Name
PPP2R1A
NCBI Description
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
Uniprot Description
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887).
Research Articles on PPP2R1A
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Product Notes
The PPP2R1A ppp2r1a (Catalog #AAA206333) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PPP2R1A can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PPP2R1A ppp2r1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAAADGD DSLYPIAVLI DELRNEDVQL RLNSIKKLST IALALGVERT RSELLPFLTD TIYDEDEVLL ALAEQLGTFT TLVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR VSSAVKAELR QYFRNLCSDD TPMVRRAAAS KLGEFAKVLE LDNVKSEIIP MFSNLASDEQ DSVRLLAVEA CVNIAQLLPQ EDLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDA NQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA. It is sometimes possible for the material contained within the vial of "PPP2R1A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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