PPP1R3B recombinant protein

PPP1R3B, 1-285aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA204808

• Gene Names: PPP1R3B; GL; PTG; PPP1R4
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: PPP1R3B; 1-285aa; Human; His tag; E Coli (Denatured); Protein phosphatase 1 regulatory subunit 3B; GL; PPP1R4; PTG; PPP1R3B recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMAVDIE YRYNCMAPSL RQERFAFKIS PKPSKPLRPC IQLSSKNEAS GMVAPAVQEK KVKKRVSFAD NQGLALTMVK VFSEFDDPLD MPFNITELLD NIVSLTTAES ESFVLDFSQP SADYLDFRNR LQADHVCLEN CVLKDKAIAG TVKVQNLAFE KTVKIRMTFD TWKSYTDFPC QYVKDTYAGS DRDTFSFDIS LPEKIQSYER MEFAVYYECN GQTYWDSNRG KNYRIIRAEL KSTQGMTKPH SGPDLGISFD QFGSPRCSYG LFPEWPSYLG YEKLGPYY
• Sequence Length: 285

• Applicable Applications for PPP1R3B recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPP1R3B recombinant protein

PPP1R3B is the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein. Recombinant human PPP1R3B protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for PPP1R3B recombinant protein

Metabolism

References

Munro S., et al. (2005) Diabetes. 51:591-598; Ota T., et al. (2004) Nat. Genet. 36:40-45;

NCBI and Uniprot Product Information

• NCBI GI #: 318037218
• NCBI GeneID: 79660
• NCBI Accession #: NP_001188258
• NCBI GenBank Nucleotide #: NM_001201329.1
• UniProt Accession #: Q86XI6; Q9H812; B3KTV3
• Molecular Weight: 35.1 kDa (308aa)
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 3B
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 3B
• NCBI Official Symbol: PPP1R3B
• NCBI Official Synonym Symbols: GL; PTG; PPP1R4
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 3B
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 3B
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R3B
• UniProt Synonym Gene Names: PPP1R4; PP1 subunit R4; PTG

NCBI Description

This gene encodes the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The encoded protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Jan 2011]

Uniprot Description

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes ().