SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PPME1 recombinant protein
PPME1 Protein
Gene Names
PPME1; PME-1
Applications
Methyltrasferase Assay
Synonyms
PPME1; PPME1 Protein; PME-1; PPME1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for PPME1 recombinant protein
Methyltrasferase Assay
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for PPME1 recombinant protein
Recombinant human mature form of PPME1 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The PPME1 gene accession number is BC003046.
Protein phosphatase methylesterase 1 (PPME1) conducts the demethylation of the catalytic subunit of protein phosphatase 2A (PP2A) and leads to inactivation of the phosphatase (1). It is demonstrated that PPME1 binds directly to the active site of PP2A and evicts the manganese ions required for the phosphatase activity (2). PPME1 is also reported to protect ERK activity in human malignant glioma (3).
Protein phosphatase methylesterase 1 (PPME1) conducts the demethylation of the catalytic subunit of protein phosphatase 2A (PP2A) and leads to inactivation of the phosphatase (1). It is demonstrated that PPME1 binds directly to the active site of PP2A and evicts the manganese ions required for the phosphatase activity (2). PPME1 is also reported to protect ERK activity in human malignant glioma (3).
Product Categories/Family for PPME1 recombinant protein
References
1. Ogris, E., et al. A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A. J Biol Chem. 274(20):14382-91, 1999.
2. Xing, Y., et al. Structural mechanism of demethylation and inactivation of protein phosphatase 2A. Cell. 133(1):154-63, 2008.
3. Puustinen, P., et al. PME-1 protects extracellular signal-regulated kinase pathway activity from protein phosphatase 2A-mediated inactivation in human malignant glioma. Cancer Res. 69(7):2870-7, 2009.
2. Xing, Y., et al. Structural mechanism of demethylation and inactivation of protein phosphatase 2A. Cell. 133(1):154-63, 2008.
3. Puustinen, P., et al. PME-1 protects extracellular signal-regulated kinase pathway activity from protein phosphatase 2A-mediated inactivation in human malignant glioma. Cancer Res. 69(7):2870-7, 2009.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43,870 Da
NCBI Official Full Name
Homo sapiens protein phosphatase methylesterase 1, mRNA
NCBI Official Synonym Full Names
protein phosphatase methylesterase 1
NCBI Official Symbol
PPME1
NCBI Official Synonym Symbols
PME-1
NCBI Protein Information
protein phosphatase methylesterase 1
Protein Family
NCBI Description
This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
Research Articles on PPME1
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Product Notes
The PPME1 (Catalog #AAA517405) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PPME1 can be used in a range of immunoassay formats including, but not limited to, Methyltrasferase Assay. Researchers should empirically determine the suitability of the PPME1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPME1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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