SDS-Page
Cyclophilin D (CYPD) Recombinant Protein | CYPD recombinant protein
Recombinant Cyclophilin D (CYPD)
Gene Names
Ppid; Ppif; Ppidl; CYP-40; 4930564J03Rik
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Cyclophilin D (CYPD); Recombinant Cyclophilin D (CYPD); CYPD recombinant protein
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
KP SNSKNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGTGS TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGPNTNGS QFFITTVPTPHLDGKHVVFG QVIKGLGVAR TLENVEVNGE KPAKLCVIAE CGELKEGDD
Applicable Applications for CYPD recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Organism Species
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
Lys9~Asp189
Tags
N-terminal His Tag
Subcellular Location
Nucleus, Cytoplasm
Traits
Freeze-dried powder
Predicted isoelectric point
6.9
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 23.5kDa
Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 23kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase D
NCBI Official Synonym Full Names
peptidylprolyl isomerase D (cyclophilin D)
NCBI Official Symbol
Ppid
NCBI Official Synonym Symbols
Ppif; Ppidl; CYP-40; 4930564J03Rik
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase D; PPIase D; rotamase D; cyclophilin 40; cyclophilin-40; cytoplasmic cyclophilin D; 40 kDa peptidyl-prolyl cis-trans isomerase
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase D
Protein Family
UniProt Gene Name
Ppid
UniProt Synonym Gene Names
PPIase D; CYP-40
UniProt Entry Name
PPID_MOUSE
Uniprot Description
PPID: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Protein type: EC 5.2.1.8; Isomerase; Nucleolus
Cellular Component: mitochondrion; cytoplasm; nucleolus; nucleus
Molecular Function: enzyme binding; FK506 binding; peptidyl-prolyl cis-trans isomerase activity; heat shock protein binding; Hsp90 protein binding; isomerase activity; peptide binding; cyclosporin A binding; transcription factor binding
Biological Process: regulation of apoptosis; protein transport; positive regulation of viral genome replication; protein peptidyl-prolyl isomerization; protein folding; positive regulation of protein secretion; transport; apoptosis; positive regulation of apoptosis; protein complex assembly; negative regulation of transcription from RNA polymerase II promoter
Research Articles on CYPD
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Product Notes
The CYPD ppid (Catalog #AAA2018468) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cyclophilin D (CYPD) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the CYPD ppid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KP SNSKNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGTGS TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGPNTNGS QFFITTVPTP HLDGKHVVFG QVIKGLGVAR TLENVEVNGE KPAKLCVIAE CGELKEGDD. It is sometimes possible for the material contained within the vial of "Cyclophilin D (CYPD), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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