Cyclophilin B (CYPB) Recombinant Protein | CYPB recombinant protein

Recombinant Cyclophilin B (CYPB)

Cat. Num. AAA2011575

• Gene Names: Ppib; CypB; Scylp
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cyclophilin B (CYPB); Recombinant Cyclophilin B (CYPB); CYPB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-NDKKKGP KVTVKVYFDF QIGDEPVGRV TFGLFGKTVP KTVDNFVALA TGEKGFGYKN SKFHRVIKDF MIQGGDFTRG DGTGGKSIYG ERFPDENFKL KHYGPGWVSM ANAGKDTNGS QFFITTVKTS WLDGKHVVFG KVLEGMDVVR KVENTKTDSR DKPLKDVIIV DCGKIEVEKP FAIAKE
• Sequence Length: 208

• Applicable Applications for CYPB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asn34~Glu216 (Accession # P24368) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 11968126
• NCBI GeneID: 64367
• NCBI Accession #: NP_071981.1
• NCBI GenBank Nucleotide #: NM_022536.2
• UniProt Accession #: P24368; O88541
• Molecular Weight: 21.8kDa
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase B
• NCBI Official Synonym Full Names: peptidylprolyl isomerase B
• NCBI Official Symbol: Ppib
• NCBI Official Synonym Symbols: CypB; Scylp
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase B; CYP-S1; PPIase B; rotamase B; S-cyclophilin; S-cyclophylin; cyclophilin B
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase B
• Protein Family: Bifunctional cytochrome P450/NADPH--P450 reductase
• UniProt Gene Name: Ppib
• UniProt Synonym Gene Names: PPIase B; SCYLP
• UniProt Entry Name: PPIB_RAT

NCBI Description

mouse homolog is an endoplasmic reticulum peptidylprolyl isomerase [RGD, Feb 2006]

Uniprot Description

PPIB: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9). OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.

Protein type: Vesicle; Isomerase; Endoplasmic reticulum; Chaperone; Secreted; Secreted, signal peptide; RNA-binding; EC 5.2.1.8; Cyclophilin

Cellular Component: focal adhesion; smooth endoplasmic reticulum; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; perinuclear region of cytoplasm; melanosome; nucleus

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; protein complex binding; peptide binding

Biological Process: nuclear transport; protein peptidyl-prolyl isomerization; protein stabilization; positive regulation of multicellular organism growth