Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Peroxisome Proliferators Activated Receptor alpha ELISA Kit | PPARalpha elisa kit
Guinea pig Peroxisome Proliferators Activated Receptor alpha ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PPARA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PPARA antibody and an PPARA-HRP conjugate. The assay sample and buffer are incubated together with PPARA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPARA concentration since PPARA from samples and PPARA-HRP conjugate compete for the anti-PPARA antibody binding site. Since the number of sites is limited, as more sites are occupied by PPARA from the sample, fewer sites are left to bind PPARA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPARA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; NFAT protein binding; DNA binding; ubiquitin conjugating enzyme binding; zinc ion binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; drug binding; transcription factor activity; lipid binding; phosphatase binding
Biological Process: circadian rhythm; epidermis development; wound healing; positive regulation of transcription, DNA-dependent; heart development; behavioral response to nicotine; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; circadian regulation of gene expression; negative regulation of blood pressure; negative regulation of glycolysis; negative regulation of protein binding; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; lipid metabolic process; fatty acid transport
Research Articles on PPARalpha
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Product Notes
The Guinea Pig PPARalpha ppara (Catalog #AAA745527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745527 ELISA Kit recognizes Guinea Pig PPARalpha. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferators Activated Receptor alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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