Human Peroxisome Proliferator Activated Receptor Gamma ELISA Kit | PPAR-gamma elisa kit

Human Peroxisome Proliferator Activated Receptor Gamma ELISA Kit

Cat. Num. AAA760630

• Reactivity: Human
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma; Human Peroxisome Proliferator Activated Receptor Gamma ELISA Kit; PPAR-gamma/PPARG/NR1C3 (Peroxisome proliferator-activated receptor gamma)/CIMT1/Nuclear receptor subfamily 1 group C member 3/peroxisome proliferator-activated receptor gamma 1/PPAR gamma/PPARG1/peroxisome proliferative activated receptor; gamma/PPARG2/PPARgamma/PPAR-gamma; PPAR-gamma elisa kit

• Reactivity: Human
• Sequence Length: 477

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PPAR-gamma elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PPAR-gamma antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PPAR-gamma antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PPAR-gamma amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PPAR-gamma can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 17978516
• Molecular Weight: 21,580 Da
• NCBI Official Full Name: peroxisome proliferator activated receptor gamma
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• UniProt Gene Name: PPARG
• UniProt Synonym Gene Names: NR1C3; PPAR-gamma
• UniProt Entry Name: PPARG_HUMAN

Uniprot Description

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: alpha-actinin binding; arachidonic acid binding; chromatin binding; DNA binding; drug binding; enzyme binding; estrogen receptor binding; identical protein binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; protein binding; protein phosphatase binding; retinoid X receptor binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription activator binding; transcription factor activity; zinc ion binding

Biological Process: brown fat cell differentiation; caspase activation; cell fate commitment; cell maturation; cellular response to insulin stimulus; diet induced thermogenesis; epithelial cell differentiation; fatty acid oxidation; G-protein coupled receptor protein signaling pathway; gene expression; glucose homeostasis; heart development; innate immune response; lipid homeostasis; lipid metabolic process; lipoprotein transport; long-chain fatty acid transport; low-density lipoprotein receptor biosynthetic process; monocyte differentiation; negative regulation of acute inflammatory response; negative regulation of cell growth; negative regulation of collagen biosynthetic process; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ regeneration; placenta development; positive regulation of fat cell differentiation; positive regulation of fatty acid oxidation; positive regulation of oligodendrocyte differentiation; positive regulation of phagocytosis, engulfment; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of blood pressure; regulation of circadian rhythm; response to caffeine; response to cold; response to estrogen stimulus; response to lipid; response to low density lipoprotein stimulus; response to mechanical stimulus; response to nutrient; response to retinoic acid; response to starvation; response to vitamin A; rhythmic process; signal transduction; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter; white fat cell differentiation

Disease: Carotid Intimal Medial Thickness 1; Diabetes Mellitus, Noninsulin-dependent; Lipodystrophy, Familial Partial, Type 3; Obesity