Phosphatidic Acid Phosphatase Type 2C Recombinant Protein | PPAP2C recombinant protein

Recombinant Phosphatidic Acid Phosphatase Type 2C (PPAP2C)

Cat. Num. AAA2123132

• Gene Names: PLPP2; LPP2; PAP-2c; PAP2-g; PPAP2C
• Applications: SDS-Page, Western Blot
• Purity: >95%
• Synonyms: Phosphatidic Acid Phosphatase Type 2C; Recombinant Phosphatidic Acid Phosphatase Type 2C (PPAP2C); PPAP2-C; LPP2; PAP-2c; PAP2-g; Lipid phosphate phosphohydrolase 2; Phosphatidate phosphohydrolase type 2c; PPAP2C recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Met1~Leu162
• Sequence Length: 288

• Applicable Applications for PPAP2C recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His and GST Tag
• Subcellular Location: Membrane
• Isoelectric Point: 8.6
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for PPAP2C recombinant protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4505977
• NCBI GeneID: 8612
• NCBI Accession #: NP_003703.1
• NCBI GenBank Nucleotide #: NP_003703.1
• UniProt Accession #: O43688
• Molecular Weight: Predicted Molecular Mass: 48.1kDa; Accurate Molecular Mass: 48kDa
• NCBI Official Full Name: phospholipid phosphatase 2 isoform 1
• NCBI Official Synonym Full Names: phospholipid phosphatase 2
• NCBI Official Symbol: PLPP2
• NCBI Official Synonym Symbols: LPP2; PAP-2c; PAP2-g; PPAP2C
• NCBI Protein Information: phospholipid phosphatase 2
• UniProt Protein Name: Lipid phosphate phosphohydrolase 2
• UniProt Gene Name: PPAP2C
• UniProt Synonym Gene Names: LPP2; PAP2-G; PAP-2c; PAP2c
• UniProt Entry Name: LPP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is PA > C-1-P > LPA > S-1-P.

Catalytic activity: A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.

Enzyme regulation: Inhibited by sphingosine, zinc ions and propanolol.

Subunit structure: Homodimer

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Found mainly in brain, pancreas and placenta.

Sequence similarities: Belongs to the PA-phosphatase related phosphoesterase family.