Human Placental protein 13 ELISA Kit | PP13 elisa kit
Human Placental protein 13 (PP13) ELISA Kit
Gene Names
LGALS13; PP13; GAL13; PLAC8
Reactivity
Human
Synonyms
Placental protein 13; Human Placental protein 13 (PP13) ELISA Kit; PP13 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LGALS13. No significant cross-reactivity or interference between Human LGALS13 and analogues was observed.
Sequence Length
139
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PP13 elisa kit
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. Galactoside-binding soluble lectin 13 has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family.By ultracentrifugation and SDS-PAGE, LGALS13 showed an apparent molecular mass of 29 to 30 kD.
Under reducing conditions, the apparent molecular mass was 16 kD, suggesting that LGALS13 is composed of 2 identical subunits held together by disulfide bonds. Western blot analysis and Ouchterlony gel diffusion were unable to detect LGALS13 in serum or in any other adult or fetal tissue examined.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LGALS13 in samples. An antibody specific for LGALS13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Under reducing conditions, the apparent molecular mass was 16 kD, suggesting that LGALS13 is composed of 2 identical subunits held together by disulfide bonds. Western blot analysis and Ouchterlony gel diffusion were unable to detect LGALS13 in serum or in any other adult or fetal tissue examined.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LGALS13 in samples. An antibody specific for LGALS13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,119 Da
NCBI Official Full Name
galactoside-binding soluble lectin 13
NCBI Official Synonym Full Names
galectin 13
NCBI Official Symbol
LGALS13
NCBI Official Synonym Symbols
PP13; GAL13; PLAC8
NCBI Protein Information
galactoside-binding soluble lectin 13
UniProt Protein Name
Galactoside-binding soluble lectin 13
Protein Family
UniProt Gene Name
LGALS13
UniProt Synonym Gene Names
PLAC8; Gal-13; PP13; Placental protein 13
NCBI Description
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family. [provided by RefSeq, Jul 2008]
Uniprot Description
LGALS13: Has lysophospholipase activity.
Protein type: EC 3.1.1.5; Phospholipase
Chromosomal Location of Human Ortholog: 19q13.2
Molecular Function: carbohydrate binding; lysophospholipase activity; protein binding
Biological Process: apoptosis; phospholipid metabolic process
Research Articles on PP13
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Product Notes
The Human PP13 lgals13 (Catalog #AAA283363) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283363 ELISA Kit recognizes Human PP13. It is sometimes possible for the material contained within the vial of "Placental protein 13, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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