Human Pituitary-specific positive transcription factor 1 ELISA Kit | POU1F1 elisa kit

Human Pituitary-specific positive transcription factor 1 (POU1F1) ELISA Kit

Cat. Num. AAA282001

• Gene Names: POU1F1; PIT1; CPHD1; GHF-1; Pit-1; POU1F1a
• Reactivity: Human
• Synonyms: Pituitary-specific positive transcription factor 1; Human Pituitary-specific positive transcription factor 1 (POU1F1) ELISA Kit; POU1F1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human POU1F1. No significant cross-reactivity or interference between Human POU1F1 and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for POU1F1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate POU1F1 in samples. An antibody specific for POU1F1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOU1F1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POU1F1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POU1F1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4505955
• NCBI GeneID: 5449
• NCBI Accession #: NP_000297.1
• NCBI GenBank Nucleotide #: NM_000306.3
• UniProt Accession #: P28069; O75757; Q15132; Q15133; Q9UD34; Q9UEL3
• Molecular Weight: 35,669 Da
• NCBI Official Full Name: pituitary-specific positive transcription factor 1 isoform alpha
• NCBI Official Synonym Full Names: POU class 1 homeobox 1
• NCBI Official Symbol: POU1F1
• NCBI Official Synonym Symbols: PIT1; CPHD1; GHF-1; Pit-1; POU1F1a
• NCBI Protein Information: pituitary-specific positive transcription factor 1
• UniProt Protein Name: Pituitary-specific positive transcription factor 1
• Protein Family: Pituitary-specific positive transcription factor
• UniProt Gene Name: POU1F1
• UniProt Synonym Gene Names: GHF1; PIT1; PIT-1; GHF-1

NCBI Description

This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GHF-1: Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'- TAAAT-3'. Belongs to the POU transcription factor family. Class- 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 3p11.2

Cellular Component: nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding transcription factor activity; transcription corepressor activity

Biological Process: B cell differentiation; cell fate specification; determination of adult lifespan; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; nuclear transport; positive regulation of cell proliferation; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of insulin-like growth factor receptor signaling pathway; regulation of transcription, DNA-templated; somatotropin secreting cell development; transcription from RNA polymerase II promoter

Disease: Pituitary Hormone Deficiency, Combined, 1