Alanine Aminotransferase Active Protein | GPT active protein
Glutamate Pyruvate Transaminase (GPT), Alanine Transaminase (ALT)
Synonyms
Alanine Aminotransferase; Glutamate Pyruvate Transaminase (GPT); Alanine Transaminase (ALT); Alanine Transaminase; ALT; GPT; GPT active protein
Host
Porcine Heart
Form/Format
Lyophilized from tris-chloride buffer, pH 7.0 with sodium chloride, EDTA, DTT, alpha-ketoglurarte, and pyridoxal-5-phosphate
Appearance
Yellow to yellow-green powder
Solubility
Clear, colorless to yellow-green solution (1 mg/mL saline)
Activity
93 units/mg (Dimension Clinical Chemistry System)
Protein
0.90 mg protein/mg (A280)
0.57 mg protein/mg (Coomassie)
0.57 mg protein/mg (Coomassie)
Specific Activity
100 U/mg protein (A280)
160 U/mg protein (Coomassie)
160 U/mg protein (Coomassie)
Unit Definition
One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
Contaminants
CPK: < 0.1%
GLDH: < 0.01%
LDH: < 0.1%
SGOT/AST: < 0.5%
Protease: < 0.02 U/mg (< 0.001 O.D./8 min.)
Ammonia: < 0.5 micromole/mg
GLDH: < 0.01%
LDH: < 0.1%
SGOT/AST: < 0.5%
Protease: < 0.02 U/mg (< 0.001 O.D./8 min.)
Ammonia: < 0.5 micromole/mg
Handling Information
Reconstitute into provided container and centrifuge to ensure full recovery.
Aliquot and freeze at -20°C after reconstitution.
Handle as a potentially hazardous substance.
Aliquot and freeze at -20°C after reconstitution.
Handle as a potentially hazardous substance.
Preparation and Storage
Store at -20°C; Protect from moisture.
Shipping: Blue Ice
Shipping: Blue Ice
Product Categories/Family for GPT active protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Approximately 100,000
NCBI Official Full Name
Alanine aminotransferase 1
UniProt Protein Name
Alanine aminotransferase 1
Protein Family
UniProt Gene Name
GPT
UniProt Synonym Gene Names
AAT1; GPT1; ALT1; GPT 1
UniProt Entry Name
ALAT1_PIG
Uniprot Description
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles ().
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Product Notes
The GPT gpt (Catalog #AAA173090) is an Active Protein produced from Porcine Heart and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Alanine Aminotransferase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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