Typical Testing Data/Standard Curve (for reference only)
Porcine thyrotropin-releasing hormone, TRH ELISA Kit | TRH elisa kit
Porcine thyrotropin-releasing hormone, TRH ELISA Kit
Gene Names
TRH; Pro-TRH
Reactivity
Porcine
Synonyms
thyrotropin-releasing hormone; TRH; Porcine thyrotropin-releasing hormone; TRH ELISA Kit; TRH elisa kit
Reactivity
Porcine
Sequence Length
260
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
5ng/L - 1000ng/L
Sensitivity
2.43ng/L
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TRH elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Thyrotropin-releasing Hormone (also known as TRH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine TRH antibody. TRH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine TRH Antibody is added and binds to TRH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine TRH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine TRH antibody. TRH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine TRH Antibody is added and binds to TRH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine TRH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,337 Da
NCBI Official Full Name
thyrotropin releasing hormone preproprotein
NCBI Official Synonym Full Names
thyrotropin releasing hormone
NCBI Official Symbol
TRH
NCBI Official Synonym Symbols
Pro-TRH
NCBI Protein Information
thyrotropin releasing hormone
UniProt Protein Name
Pro-thyrotropin-releasing hormone
Protein Family
UniProt Gene Name
TRH
UniProt Synonym Gene Names
Pro-TRH; TRH; TRF
Uniprot Description
Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems.
Research Articles on TRH
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Product Notes
The Porcine TRH trh (Catalog #AAA160623) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA160623 ELISA Kit recognizes Porcine TRH. It is sometimes possible for the material contained within the vial of "thyrotropin-releasing hormone, TRH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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