Rat NUP160 ELISA Kit | NUP160 elisa kit

Rat NUP160 (Nucleoporin 160kDa) ELISA Kit

Cat. Num. AAA2504948

• Reactivity: Rat
• Synonyms: NUP160; Rat NUP160 (Nucleoporin 160kDa) ELISA Kit; NUP160 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat NUP160. No significant cross-reactivity or interference between Rat NUP160 and analogues was observed.
• Sequence Length: 1436

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUP160 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat NUP160 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NUP160. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NUP160 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NUP160, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NUP160. You can calculate the concentration of NUP160 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 54859722
• NCBI GeneID: 23279
• NCBI Accession #: NP_056046.1
• NCBI GenBank Nucleotide #: NM_015231.1
• UniProt Accession #: Q12769; Q08AD3; Q7Z5X6; Q96GB3; Q9H660; B4DYE8; B4E2J9
• Molecular Weight: 55,554 Da
• NCBI Official Full Name: nuclear pore complex protein Nup160
• NCBI Official Synonym Full Names: nucleoporin 160kDa
• NCBI Official Symbol: NUP160
• NCBI Protein Information: nuclear pore complex protein Nup160; 160 kDa nucleoporin; nucleoporin 160kD; nucleoporin Nup160
• UniProt Protein Name: Nuclear pore complex protein Nup160
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP160
• UniProt Synonym Gene Names: KIAA0197; NUP120
• UniProt Entry Name: NU160_HUMAN

NCBI Description

NUP160 is 1 of up to 60 proteins that make up the 120-MD nuclear pore complex, which mediates nucleoplasmic transport.[supplied by OMIM, Apr 2004]

Uniprot Description

NUP160: Involved in poly(A)+ RNA transport. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: kinetochore; nuclear pore; nuclear envelope; cytosol

Molecular Function: protein binding; nucleocytoplasmic transporter activity

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; mRNA export from nucleus; protein transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport