Porcine Soluble Cluster of differentiation 40 ligand (sCD40L) ELISA Kit | sCD40L elisa kit

Porcine Soluble Cluster of differentiation 40 ligand (sCD40L) ELISA KIT

Cat. Num. AAA267518

• Gene Names: SLC9C1; NHE; SLC9A10; sperm-NHE
• Reactivity: Porcine
• Synonyms: Soluble Cluster of differentiation 40 ligand (sCD40L); Porcine Soluble Cluster of differentiation 40 ligand (sCD40L) ELISA KIT; sCD40L elisa kit

• Reactivity: Porcine
• Sequence Length: 1177

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for sCD40L elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine sCD40L monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 158563886
• NCBI GeneID: 285335
• Molecular Weight: 129,365 Da
• NCBI Official Full Name: Sodium/hydrogen exchanger 10
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
• NCBI Official Symbol: SLC9C1
• NCBI Official Synonym Symbols: NHE; SLC9A10; sperm-NHE
• NCBI Protein Information: sodium/hydrogen exchanger 10; sNHE; NHE-10; Na(+)/H(+) exchanger 10; solute carrier family 9 member C1; solute carrier family 10 member 10; solute carrier family 9, member 10; solute carrier family 9, isoform 10; sperm-specific Na(+)/H(+) exchanger; sperm-specific sodium proton exchanger
• UniProt Protein Name: Sodium/hydrogen exchanger 10
• UniProt Gene Name: SLC9C1
• UniProt Synonym Gene Names: SLC9A10; NHE-10; sNHE
• UniProt Entry Name: SL9C1_HUMAN

NCBI Description

SLC9A10 is a member of the sodium-hydrogen exchanger (NHE) family (see SLC9A1, MIM 107310) and is required for male fertility and sperm motility (Wang et al., 2003 [PubMed 14634667]).[supplied by OMIM, Apr 2009]

Uniprot Description

NHE10: Sperm-specific sodium/hydrogen exchanger involved in intracellular pH regulation of spermatozoa. Required for sperm motility and fertility. Involved in sperm cell hyperactivation, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Required for the expression and bicarbonate regulation of the soluble adenylyl cyclase (sAC). Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: solute:hydrogen antiporter activity

Biological Process: sperm motility; multicellular organismal development; sodium ion transport; spermatogenesis; cell differentiation; transmembrane transport