Porcine Phospholipase A2, Pancreas (pPLA2) ELISA Kit | pPLA2 elisa kit

Pig Phospholipase A2, Pancreas (pPLA2) ELISA Kit

Cat. Num. AAA2705259

• Gene Names: PLA2G1B; PLA2; PLA2A; PPLA2
• Reactivity: Porcine
• Synonyms: Phospholipase A2; Pancreas (pPLA2); Pig Phospholipase A2; Pancreas (pPLA2) ELISA Kit; PLA2G1B; PLA2A; PPLA2; Group IB; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B; pPLA2 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Pancreas (pPLA2).; No significant cross-reactivity or interference between Phospholipase A2, Pancreas (pPLA2) and analogues was observed.
• Sequence Length: 148

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 13.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Pancreas (pPLA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Pancreas (pPLA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for pPLA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Pancreas (pPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Pancreas (pPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Pancreas (pPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Pancreas (pPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505847
• NCBI GeneID: 5319
• NCBI Accession #: NP_000919.1
• NCBI GenBank Nucleotide #: NM_000928.2
• UniProt Accession #: P04054; Q3KPI1; B2R4H5
• Molecular Weight: 16,360 Da
• NCBI Official Full Name: phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2 group IB
• NCBI Official Symbol: PLA2G1B
• NCBI Official Synonym Symbols: PLA2; PLA2A; PPLA2
• NCBI Protein Information: phospholipase A2
• UniProt Protein Name: Phospholipase A2
• UniProt Gene Name: PLA2G1B
• UniProt Synonym Gene Names: PLA2; PLA2A; PPLA2

NCBI Description

This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]

Uniprot Description

PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: DNA replication; EC 3.1.1.4; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Phospholipase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell surface; cytosol; extracellular region; extracellular space; secretory granule

Molecular Function: bile acid binding; calcium ion binding; calcium-dependent phospholipase A2 activity; phospholipase A2 activity; receptor binding

Biological Process: actin filament organization; activation of MAPK activity; activation of phospholipase A2 activity; antibacterial humoral response; arachidonic acid secretion; cellular response to insulin stimulus; defense response to Gram-positive bacterium; fatty acid biosynthetic process; glucose transport; innate immune response in mucosa; interleukin-8 production; leukotriene biosynthetic process; multicellular organismal lipid catabolic process; neutrophil chemotaxis; neutrophil mediated immunity; phosphatidic acid biosynthetic process; phosphatidylcholine metabolic process; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of immune response; positive regulation of NF-kappaB transcription factor activity; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; signal transduction