Rabbit anti-Human VPS52 Polyclonal Antibody | anti-VPS52 antibody

VPS52, CT (VPS52, SACM2L, Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein) (Azide free) (HRP)

Cat. Num. AAA6362778

• Gene Names: VPS52; ARE1; SAC2; SACM2L; RP5-1033B10; dJ1033B10.5
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: VPS52; Polyclonal Antibody; CT (VPS52; SACM2L; Vacuolar protein sorting-associated protein 52 homolog; SAC2 suppressor of actin mutations 2-like protein) (Azide free) (HRP); SAC2 suppressor of actin mutations 2-like protein; anti-VPS52 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-VPS52 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-641 amino acids from the C-terminal region of human VPS52.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(VPS52 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the VPS52 antibody detected the VPS52 protein (arrow).)

Immunohistochemistry (IHC)

(VPS52 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VPS52 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(VPS52 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of VPS52 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-VPS52 antibody

This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.

Product Categories/Family for anti-VPS52 antibody

Antibodies; Actin Related Proteins

References

Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009): Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009). Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008). Liewen, H., et al. Exp. Cell Res. 306(1):24-34(2005). Mungall, A.J., et al. Nature 425(6960):805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 73747799
• NCBI GeneID: 6293
• NCBI Accession #: NP_072047.4
• NCBI GenBank Nucleotide #: NM_022553.4
• UniProt Accession #: Q8N1B4; Q53GR4; Q5JPA0; Q5SQW1; Q8IUN6; Q9NPT5; A2BF38; B0UZZ4
• Molecular Weight: 82,221 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 52 homolog
• NCBI Official Synonym Full Names: vacuolar protein sorting 52 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS52
• NCBI Official Synonym Symbols: ARE1; SAC2; SACM2L; RP5-1033B10; dJ1033B10.5
• NCBI Protein Information: vacuolar protein sorting-associated protein 52 homolog; SAC2 suppressor of actin mutations 2-like protein
• UniProt Protein Name: Vacuolar protein sorting-associated protein 52 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS52
• UniProt Synonym Gene Names: SACM2L
• UniProt Entry Name: VPS52_HUMAN

NCBI Description

This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Ref.6 Ref.7

Subunit structure: Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts with RAB6A and STX10. Ref.6 Ref.8

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein Ref.6 Ref.7.

Sequence similarities: Belongs to the VPS52 family.

Sequence caution: The sequence CAI95619.1 differs from that shown. Reason: Erroneous gene model prediction.