Porcine Metalloproteinase 8 (MMP-8) ELISA Kit | MMP-8 elisa kit

Porcine Metalloproteinase 8 (MMP-8) ELISA kit

Cat. Num. AAA267821

• Gene Names: MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
• Reactivity: Porcine
• Synonyms: Metalloproteinase 8 (MMP-8); Porcine Metalloproteinase 8 (MMP-8) ELISA kit; MMP-8 elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 457

• Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MMP-8 concentration
• Assay Type: Sandwich
• Detection Range: 5000 pg/ml-78 pg/ml
• Sensitivity: 20 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP-8 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine MMP-8 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 1209010
• NCBI GeneID: 4325
• Molecular Weight: 52,515 Da
• NCBI Official Full Name: metalloproteinase
• NCBI Official Synonym Full Names: matrix metallopeptidase 16 (membrane-inserted)
• NCBI Official Symbol: MMP16
• NCBI Official Synonym Symbols: MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
• NCBI Protein Information: matrix metalloproteinase-16; MMP-16; MT3MMP; MTMMP3; MT-MMP 3; Putative transmembrane protein C8orf57; membrane-type matrix metalloproteinase 3; membrane-type-3 matrix metalloproteinase
• UniProt Protein Name: Matrix metalloproteinase-16
• UniProt Gene Name: MMP16
• UniProt Synonym Gene Names: MMPX2; MMP-16; MT-MMP 3; MTMMP3; MT3-MMP; MT3MMP
• UniProt Entry Name: MMP16_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]

Uniprot Description

MMP16: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: proteinaceous extracellular matrix; cell surface; Golgi lumen; integral to plasma membrane; plasma membrane

Molecular Function: zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding

Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; embryonic cranial skeleton morphogenesis; proteolysis; endochondral ossification