Porcine Low Density Lipoprotein (LDL) ELISA Kit | LDL elisa kit

Porcine Low Density Lipoprotein (LDL) ELISA Kit

Cat. Num. AAA265058

• Reactivity: Porcine
• Synonyms: Low Density Lipoprotein (LDL); Porcine Low Density Lipoprotein (LDL) ELISA Kit; LDL elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 76

• Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LDL concentration
• Assay Type: Sandwich
• Detection Range: 200 ng/ml-3.12 ng/ml
• Sensitivity: 0.6 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDL elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine LDL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 306411097
• Molecular Weight: 8,219 Da
• NCBI Official Full Name: low density lipoprotein, partial
• UniProt Protein Name: Low density lipoprotein
• UniProt Gene Name: LDLR
• UniProt Entry Name: E1B4T0_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; lysosome; early endosome; late endosome; plasma membrane; endosome membrane; coated pit; receptor complex; external side of plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; calcium ion binding; clathrin heavy chain binding; glycoprotein binding

Biological Process: lipoprotein catabolic process; phototransduction, visible light; receptor-mediated endocytosis; cholesterol metabolic process; cholesterol homeostasis; cholesterol transport; viral reproduction; cholesterol absorption; phospholipid transport; endocytosis; lipoprotein metabolic process; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial