Porcine I-TAC ELISA Kit | I-TAC elisa kit

Porcine I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit

Cat. Num. AAA2506425

• Gene Names: CXCL11; IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• Reactivity: Porcine
• Synonyms: I-TAC; Porcine I-TAC (Interferon Inducible T-cell Alpha Chemoattractant) ELISA Kit; I-TAC elisa kit

• Reactivity: Porcine
• Specificity: This kit recognizes natural and recombinantPorcineI-TAC. No significant cross-reactivity or interference between PorcineI-TAC and analogues was observed.
• Sequence Length: 73

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for I-TAC elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of PorcineI-TACconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to I-TAC. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for I-TACand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain I-TAC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of I-TAC.You can calculate the concentration of I-TACin the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 14030443
• NCBI GeneID: 6373
• UniProt Accession #: O14625; Q53YA3; Q92840
• Molecular Weight: 10,365 Da
• NCBI Official Full Name: I-TAC, partial
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 11
• NCBI Official Symbol: CXCL11
• NCBI Official Synonym Symbols: IP9; H174; IP-9; b-R1; I-TAC; SCYB11; SCYB9B
• NCBI Protein Information: C-X-C motif chemokine 11; beta-R1; interferon gamma-inducible protein 9; interferon-inducible T-cell alpha chemoattractant; small inducible cytokine B11; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; small-inducible cytokine B11
• UniProt Protein Name: C-X-C motif chemokine 11
• UniProt Gene Name: CXCL11
• UniProt Synonym Gene Names: ITAC; SCYB11; SCYB9B; IP-9; I-TAC
• UniProt Entry Name: CXL11_HUMAN

NCBI Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC. This antimicrobial gene is a CXC member of the chemokine superfamily. Its encoded protein induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

CXCL11: Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q21.2

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cAMP metabolic process; signal transduction; inflammatory response; chemotaxis; regulation of cell proliferation