Porcine GRbeta ELISA Kit | GRbeta elisa kit
Porcine GRbeta (Glucocorticoid Receptor Beta) ELISA Kit
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GRbeta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GRbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GRbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GRbeta. You can calculate the concentration of GRbeta in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
Uniprot Description
GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.
Protein type: DNA-binding; Mitochondrial; Nuclear receptor; Transcription factor
Chromosomal Location of Human Ortholog: 5q31.3
Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; nucleus; cytosol
Molecular Function: protein dimerization activity; protein binding; glucocorticoid receptor activity; zinc ion binding; transcription factor activity; steroid binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; adrenal gland development; maternal behavior; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid mediated signaling; glucocorticoid metabolic process; regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; positive regulation of neuron apoptosis; gene expression; regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter
Disease: Glucocorticoid Resistance, Generalized
