Porcine Granulocyte-Macrophage Colony Stimulating Factor, GM-CSF ELISA Kit | GM-CSF elisa kit

Porcine Granulocyte-Macrophage Colony Stimulating Factor, GM-CSF ELISA Kit

Cat. Num. AAA1600874

• Gene Names: CSF2; CSF; GMCSF
• Reactivity: Porcine
• Synonyms: Granulocyte-Macrophage Colony Stimulating Factor; GM-CSF; Porcine Granulocyte-Macrophage Colony Stimulating Factor; GM-CSF ELISA Kit; GM-CSF elisa kit

• Reactivity: Porcine
• Sequence Length: 144

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 2ng/L - 600ng/L
• Sensitivity: 1.12ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GM-CSF elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Porcine Granulocyte-Macrophage Colony Stimulating Factor (also known as GM-CSF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine GM-CSF antibody. GM-CSF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine GM-CSF Antibody is added and binds to GM-CSF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GM-CSF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine GM-CSF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 27437030
• NCBI GeneID: 1437
• NCBI Accession #: NP_000749.2
• NCBI GenBank Nucleotide #: NM_000758.3
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: CSF; GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.