Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with USP51 Antibody (Center). MBS9214039 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit anti-Human USP51 Polyclonal Antibody | anti-USP51 antibody
USP51 Antibody (Center)
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
USP51; Polyclonal Antibody; USP51 Antibody (Center); Ubiquitin carboxyl-terminal hydrolase 51; Deubiquitinating enzyme 51; Ubiquitin thioesterase 51; Ubiquitin-specific-processing protease 51; anti-USP51 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This USP51 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-307 amino acids from the Central region of human USP51.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
278-307
Sequence Length
711
Applicable Applications for anti-USP51 antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)
Application Notes
IF~~1:25
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with USP51 Antibody (Center). MBS9214039 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with USP51 Antibody (Center). MBS9214039 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(USP51 Antibody (Center) western blot analysis in HepG2,MCF-7 cell line lysates (35ug/lane).This demonstrates the USP51 antibody detected the USP51 protein (arrow).)
Western Blot (WB)
(USP51 Antibody (Center) western blot analysis in HepG2,MCF-7 cell line lysates (35ug/lane).This demonstrates the USP51 antibody detected the USP51 protein (arrow).)
Product Categories/Family for anti-USP51 antibody
References
Quesada V., et al. Biochem. Biophys. Res. Commun. 314:54-62(2004).
Ross M.T., et al. Nature 434:325-337(2005).
Puente X.S., et al. Genome Res. 14:609-622(2004).
Ross M.T., et al. Nature 434:325-337(2005).
Puente X.S., et al. Genome Res. 14:609-622(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79756
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 51
NCBI Official Synonym Full Names
ubiquitin specific peptidase 51
NCBI Official Symbol
USP51
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 51
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 51
Protein Family
UniProt Gene Name
USP51
UniProt Entry Name
UBP51_HUMAN
Uniprot Description
USP51: Belongs to the peptidase C19 family.
Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: Xp11.21
Molecular Function: zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription, DNA-dependent; histone deubiquitination
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Product Notes
The USP51 usp51 (Catalog #AAA9214039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 278-307. The USP51 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP51 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the USP51 usp51 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP51, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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