Porcine Connexin 43 (CX43) ELISA Kit | CX43 elisa kit

Porcine Connexin 43 (CX43) ELISA Kit

Cat. Num. AAA456621

• Gene Names: GJA1; CX43
• Reactivity: Porcine
• Synonyms: Connexin 43 (CX43); Porcine Connexin 43 (CX43) ELISA Kit; CX43 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of CX43. No significant cross-reactivity or interference between CX43 and analogues was observed.
• Sequence Length: 125

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.16ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CX43 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CX43 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for CX43 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CX43 in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CX43. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CX43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 346644748
• NCBI GeneID: 100518636
• NCBI Accession #: NP_001231141.1
• NCBI GenBank Nucleotide #: NM_001244212.1
• UniProt Accession #: Q9GJX4; Q29101
• Molecular Weight: 13,898 Da
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Symbol: GJA1
• NCBI Official Synonym Symbols: CX43
• NCBI Protein Information: gap junction alpha-1 protein
• UniProt Protein Name: Connexin 43
• UniProt Gene Name: connexin 43