Human Connexin 43 (CX43) ELISA Kit | CX43 elisa kit

Human Connexin 43 (CX43) ELISA Kit

Cat. Num. AAA450847

• Gene Names: GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; HLHS1; PPKCA
• Reactivity: Human
• Synonyms: Connexin 43 (CX43); Human Connexin 43 (CX43) ELISA Kit; ODDD; GJAL; GJA1; GJ-A1; Gap junction 43 kDa heart protein; Gap Junction Protein Alpha 1; Oculodentodigital Dysplasia; Syndactyly Type III; CX43 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CX43. No significant cross-reactivity or interference between CX43 and analogues was observed.
• Sequence Length: 382

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CX43 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CX43 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX43 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CX43 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CX43. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CX43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504001
• NCBI GeneID: 2697
• NCBI Accession #: NP_000156.1
• NCBI GenBank Nucleotide #: NM_000165.4
• UniProt Accession #: P17302; Q6FHU1; Q9Y5I8; B2R5U9
• Molecular Weight: 43,008 Da
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein alpha 1
• NCBI Official Symbol: GJA1
• NCBI Official Synonym Symbols: HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; HLHS1; PPKCA
• NCBI Protein Information: gap junction alpha-1 protein
• UniProt Protein Name: Gap junction alpha-1 protein
• UniProt Gene Name: GJA1
• UniProt Synonym Gene Names: GJAL; Cx43

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]

Uniprot Description

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles ().