Porcine Ceruloplasmin (CP) ELISA Kit | CP elisa kit

Porcine Ceruloplasmin (CP) ELISA Kit

Cat. Num. AAA267167

• Gene Names: CP; CP-2
• Reactivity: Porcine
• Synonyms: Ceruloplasmin (CP); Porcine Ceruloplasmin (CP) ELISA Kit; CP elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 1065

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine CP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557485
• NCBI GeneID: 1356
• NCBI Accession #: NP_000087.1
• NCBI GenBank Nucleotide #: NM_000096.3
• Molecular Weight: 122,205 Da
• NCBI Official Full Name: ceruloplasmin
• NCBI Official Synonym Full Names: ceruloplasmin (ferroxidase)
• NCBI Official Symbol: CP
• NCBI Official Synonym Symbols: CP-2
• NCBI Protein Information: ceruloplasmin
• UniProt Protein Name: Ceruloplasmin
• Protein Family: Ceruloplasmin
• UniProt Gene Name: CP
• UniProt Entry Name: CERU_HUMAN

NCBI Description

The protein encoded by this gene is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II)transferrin to Fe(III) transferrin. Mutations in this gene cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CP: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense. Defects in CP are the cause of aceruloplasminemia (ACERULOP). It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances. Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. Belongs to the multicopper oxidase family.

Protein type: Oxidoreductase; EC 1.16.3.1; Secreted; Secreted, signal peptide; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 3q23-q25

Cellular Component: extracellular space; lysosomal membrane; extracellular region

Molecular Function: ferroxidase activity; copper ion binding; chaperone binding

Biological Process: cellular iron ion homeostasis; copper ion transport; transmembrane transport

Disease: Aceruloplasminemia