Typical Testing Data/Standard Curve (for reference only)
Porcine Beta-synuclein (SNCB) ELISA Kit | SNCB elisa kit
Porcine Beta-synuclein (SNCB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SNCB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SNCB antibody and an SNCB-HRP conjugate. The assay sample and buffer are incubated together with SNCB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNCB concentration since SNCB from samples and SNCB-HRP conjugate compete for the anti-SNCB antibody binding site. Since the number of sites is limited, as more sites are occupied by SNCB from the sample, fewer sites are left to bind SNCB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNCB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is highly homologous to alpha-synuclein. These proteins are abundantly expressed in the brain and putatively inhibit phospholipase D2 selectively. The encoded protein, which may play a role in neuronal plasticity, is abundant in neurofibrillary lesions of patients with Alzheimer disease. This protein has been shown to be highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson disease; however, no direct relation to Parkinson disease has been established. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SNCB: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May play a role in neuronal plasticity, is abundant in neurofibrillary lesions of Alzheimer's patients. Highly expressed in the substantia nigra of the brain, a region of neuronal degeneration in patients with Parkinson disease.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: nucleoplasm; Golgi apparatus; growth cone; cell soma; mitochondrion; cytoplasm; terminal button; inclusion body
Molecular Function: phospholipase inhibitor activity; beta-tubulin binding; calcium ion binding; alpha-tubulin binding
Biological Process: synaptic transmission; negative regulation of catalytic activity; synapse organization and biogenesis; negative regulation of neuron apoptosis; dopamine metabolic process
Disease: Dementia, Lewy Body
Research Articles on SNCB
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Product Notes
The Porcine SNCB sncb (Catalog #AAA7242220) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7242220 ELISA Kit recognizes Porcine SNCB. It is sometimes possible for the material contained within the vial of "Beta-synuclein (SNCB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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