PTH / parathyroid hormone Recombinant Protein | PTH recombinant protein

Recombinant Human PTH / parathyroid hormone Protein (aa 32-65, GST tag)

Cat. Num. AAA2545381

• Gene Names: ALDOB; ALDB; ALDO2
• Purity: > 96 % as determined by SDS-PAGE
• Synonyms: PTH / parathyroid hormone; Recombinant Human PTH / parathyroid hormone Protein (aa 32-65; GST tag); PTH1; PTH recombinant protein

• Host: E Coli
• Purity/Purification: > 96 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 364

• Application Notes: The recombinant human PTH/GST chimera consists of 265 amino acids and has a predicted molecular mass of 31 kDa. It migrates as an approxiamtely 30 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PTH recombinant protein

Background: Parathyroid hormone (PTH), parathormone or parathyrin, is secreted by the chief cells of the parathyroid glands as a polypeptide. PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Parathyroid hormone (PTH) has been proved to play a pivotal role in maintaining myocardial contractility as well as effective natriuresis, and possible pathogenic mechanisms contributing to heart failure secondary to hypocalcemia and hypoparathyroidism. With the increased population of preosteoblastic lineages and the osteoblastic activation, Parathyroid hormone (PTH) drives anabolism in bone. Experiments have recently reported that PTH affects bone cells in a dual pathway - mediating osteoblastic (preosteoblastic) activities or osteocytic synthesis of sclerostin. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps.

Description: A DNA sequence encoding the human PTH (P05062) (Ser 32-Phe 65) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 40354205
• NCBI GeneID: 229
• NCBI Accession #: NP_000026.2
• NCBI GenBank Nucleotide #: NM_000035.3
• UniProt Accession #: P05062; Q13741; Q13742; Q5T7D6
• Molecular Weight: 39,473 Da
• NCBI Official Full Name: fructose-bisphosphate aldolase B
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate B
• NCBI Official Symbol: ALDOB
• NCBI Official Synonym Symbols: ALDB; ALDO2
• NCBI Protein Information: fructose-bisphosphate aldolase B
• UniProt Protein Name: Fructose-bisphosphate aldolase B
• Protein Family: Parathyroid hormone
• UniProt Gene Name: ALDOB
• UniProt Synonym Gene Names: ALDB

NCBI Description

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

Uniprot Description

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: cytosol; microtubule organizing center

Molecular Function: ATPase binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; identical protein binding; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose metabolic process; gluconeogenesis; glycolysis; NADH oxidation; positive regulation of ATPase activity

Disease: Fructose Intolerance, Hereditary