Typical Testing Data/Standard Curve (for reference only)
Porcine Aldehyde dehydrogenase family 3 member B1 (ALDH3B1) ELISA Kit | ALDH3B1 elisa kit
Porcine Aldehyde dehydrogenase family 3 member B1 (ALDH3B1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ALDH3B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALDH3B1 antibody and an ALDH3B1-HRP conjugate. The assay sample and buffer are incubated together with ALDH3B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH3B1 concentration since ALDH3B1 from samples and ALDH3B1-HRP conjugate compete for the anti-ALDH3B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH3B1 from the sample, fewer sites are left to bind ALDH3B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH3B1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldehyde dehydrogenase protein family. Aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The encoded protein is able to oxidize long-chain fatty aldehydes in vitro, and may play a role in protection from oxidative stress. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
ALDH3B1: Oxidizes medium and long chain saturated and unsaturated aldehydes. Metabolizes also benzaldehyde. Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde. May not metabolize short chain aldehydes. May use both NADP(+) and NAD(+) as cofactors. May have a protective role against the cytotoxicity induced by lipid peroxidation. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - histidine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tyrosine; EC 1.2.1.5; Amino Acid Metabolism - phenylalanine
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: cytoplasm; plasma membrane; cytosol; vesicle
Molecular Function: aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity
Biological Process: aldehyde catabolic process; ethanol catabolic process; alcohol metabolic process; lipid metabolic process
Research Articles on ALDH3B1
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Product Notes
The Porcine ALDH3B1 aldh3b1 (Catalog #AAA7211522) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211522 ELISA Kit recognizes Porcine ALDH3B1. It is sometimes possible for the material contained within the vial of "Aldehyde dehydrogenase family 3 member B1 (ALDH3B1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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