Porcine adrenergic alpha-1A receptor (ADRA1A) ELISA Kit | ADRA1A elisa kit

Porcine adrenergic alpha-1A receptor (ADRA1A) ELISA Kit

Cat. Num. AAA262017

• Gene Names: ADRA1A; ADRA1C; ADRA1L1; ALPHA1AAR
• Reactivity: Porcine
• Synonyms: adrenergic alpha-1A receptor (ADRA1A); Porcine adrenergic alpha-1A receptor (ADRA1A) ELISA Kit; ADRA1A elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 466

• Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ADRA1A concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADRA1A elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine ADRA1A monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 111118992
• NCBI GeneID: 148
• NCBI Accession #: NP_000671.2
• NCBI GenBank Nucleotide #: NM_000680.2
• Molecular Weight: 40,638 Da
• NCBI Official Full Name: alpha-1A adrenergic receptor isoform 1
• NCBI Official Synonym Full Names: adrenoceptor alpha 1A
• NCBI Official Symbol: ADRA1A
• NCBI Official Synonym Symbols: ADRA1C; ADRA1L1; ALPHA1AAR
• NCBI Protein Information: alpha-1A adrenergic receptor; alpha-1A adrenoceptor; alpha-1A adrenoreceptor; G protein coupled receptor; alpha-1C adrenergic receptor; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; adrenergic, alpha-1A-, receptor variant 11
• UniProt Protein Name: Alpha-1A adrenergic receptor
• Protein Family: Alpha-1A adrenergic receptor
• UniProt Gene Name: ADRA1A
• UniProt Synonym Gene Names: ADRA1C; Alpha-1A adrenoceptor
• UniProt Entry Name: ADA1A_HUMAN

NCBI Description

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRA1A: This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; Z disc; nucleus

Molecular Function: protein heterodimerization activity; alpha1-adrenergic receptor activity

Biological Process: apoptosis; response to hormone stimulus; positive regulation of systemic arterial blood pressure; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; signal transduction; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; smooth muscle contraction; positive regulation of MAPKKK cascade; cell-cell signaling; positive regulation of action potential; positive regulation of smooth muscle contraction; cell growth; aging; adult heart development; response to drug; positive regulation of the force of heart contraction by epinephrine-norepinephrine; positive regulation of synaptic transmission, GABAergic; micturition; G-protein coupled receptor protein signaling pathway; organ growth; negative regulation of heart rate in baroreceptor response to increased systemic arterial blood pressure; negative regulation of Rho protein signal transduction; phospholipase C activation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of heart rate by epinephrine-norepinephrine