Human Paraoxonase 1 (PON1) ELISA Kit | PON1 elisa kit

Human Paraoxonase 1 (PON1) ELISA Kit

Cat. Num. AAA705307

• Gene Names: PON1; ESA; PON; MVCD5
• Reactivity: Human
• Synonyms: Paraoxonase 1 (PON1); Human Paraoxonase 1 (PON1) ELISA Kit; Human Paraoxonase 1 (PON1); PON1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PON. No significant cross-reactivity or interference between human PON and analogues was observed.
• Sequence Length: 355

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 mIU/ml-2000 mIU/ml
• Sensitivity: < 7.81 mIU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PON1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PON has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PON present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PON is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PON bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Categories/Family for PON1 elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 19923106
• NCBI GeneID: 5444
• NCBI Accession #: NP_000437.3
• NCBI GenBank Nucleotide #: NM_000446.5
• UniProt Accession #: P27169; Q16052; Q6B0J6; Q9UCB1; B2RA40
• Molecular Weight: 39,731 Da
• NCBI Official Full Name: serum paraoxonase/arylesterase 1
• NCBI Official Synonym Full Names: paraoxonase 1
• NCBI Official Symbol: PON1
• NCBI Official Synonym Symbols: ESA; PON; MVCD5
• NCBI Protein Information: serum paraoxonase/arylesterase 1; K-45; PON 1; esterase A; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1
• UniProt Protein Name: Serum paraoxonase/arylesterase 1
• Protein Family: Serum paraoxonase/arylesterase
• UniProt Gene Name: PON1
• UniProt Synonym Gene Names: PON; PON 1
• UniProt Entry Name: PON1_HUMAN

NCBI Description

The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Ref.18 Ref.20

Catalytic activity: A phenyl acetate + H2O = a phenol + acetate. Ref.3 Ref.15 Ref.16 Ref.20 Ref.24An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol. Ref.3 Ref.15 Ref.16 Ref.20 Ref.24An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine. Ref.3 Ref.15 Ref.16 Ref.20 Ref.24

Cofactor: Binds 2 calcium ions per subunit.

Subunit structure: Homodimer. Heterooligomer with phosphate-binding protein (HPBP). Interacts with CLU. Ref.14 Ref.20 Ref.22

Subcellular location: Secreted › extracellular space.

Tissue specificity: Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. Ref.13 Ref.14

Post-translational modification: Glycosylated.The signal sequence is not cleaved.Present in two forms, form B contains a disulfide bond, form A does not.

Polymorphism: The allelic form of the enzyme with Gln-192 (allozyme A) hydrolyzes paraoxon with a low turnover number and the one with Arg-192 (allozyme B) with a high turnover number.

Involvement in disease: Microvascular complications of diabetes 5 (MVCD5) [MIM:612633]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Homozygosity for the Leu-55 allele is strongly associated with the development of retinal disease in diabetic patients.

Miscellaneous: The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces.

Sequence similarities: Belongs to the paraoxonase family.