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Western Blot (WB)
(Western blot analysis of ZWINT antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ZW10 Interactor 1 Polyclonal Antibody | anti-ZWINT1 antibody
ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174) (AP)
Gene Names
ZWINT; SIP30; ZWINT1; KNTC2AP; HZwint-1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
ZW10 Interactor 1; Polyclonal Antibody; ZW10 Interactor 1 (ZW10-interacting Protein 1; ZWINT 1; ZWINT1; ZWINT-1; ZW10 Interactor; ZWINT; Human ZW10 Interacting Protein 1; HZwint 1; HZwint-1; KNTC2AP; MGC117174) (AP); anti-ZWINT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ZWINT. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-ZWINT1 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
ELISA: 1:1,000
IHC: 1:50-1:100
WB: 1:50-1:100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:50-1:100
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide between amino acids 66-96 in the center region of human ZWINT
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ZWINT antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ZWINT antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(Western blot analysis of ZWINT (arrow) using rabbit polyclonal ZWINT Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.)
Western Blot (WB)
(Western blot analysis of ZWINT (arrow) using rabbit polyclonal ZWINT Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.)
Product Categories/Family for anti-ZWINT1 antibody
References
1. Famulski, J.K., J. Cell Biol. 180(3), 507-520 (2008). 2. Kops, G.J., J. Cell Biol. 169(1), 49-60 (2005). 3. Wang, H., J. Biol. Chem. 279(52), 54590-54598 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,544 Da
NCBI Official Full Name
ZW10 interactor isoform a
NCBI Official Synonym Full Names
ZW10 interacting kinetochore protein
NCBI Official Symbol
ZWINT
NCBI Official Synonym Symbols
SIP30; ZWINT1; KNTC2AP; HZwint-1
NCBI Protein Information
ZW10 interactor; SNAP25 interacting protein of 30 kDa; ZW10 interactor, kinetochore protein; human ZW10 interacting protein-1; zwint-1
UniProt Protein Name
ZW10 interactor
UniProt Gene Name
ZWINT
UniProt Synonym Gene Names
Zwint-1
UniProt Entry Name
ZWINT_HUMAN
Uniprot Description
ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 10q21-q22
Cellular Component: cytoplasm; cytosol; kinetochore
Molecular Function: protein binding; protein N-terminus binding
Biological Process: establishment of cellular localization; mitotic cell cycle checkpoint; mitotic sister chromatid segregation; sister chromatid cohesion
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Product Notes
The ZWINT1 zwint (Catalog #AAA6506675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZW10 Interactor 1 (ZW10-interacting Protein 1, ZWINT 1, ZWINT1, ZWINT-1, ZW10 Interactor, ZWINT, Human ZW10 Interacting Protein 1, HZwint 1, HZwint-1, KNTC2AP, MGC117174) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ZW10 Interactor 1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 ELISA: 1:1,000 IHC: 1:50-1:100 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZWINT1 zwint for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZW10 Interactor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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