Rabbit anti-Human ZP4 Polyclonal Antibody | anti-ZP4 antibody

ZP4, CT (ZP4, ZPB, Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4) (Biotin)

Cat. Num. AAA6368111

• Gene Names: ZP4; ZBP; ZP1; ZPB; Zp-4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZP4; Polyclonal Antibody; CT (ZP4; ZPB; Zona pellucida sperm-binding protein 4; Zona pellucida glycoprotein 4; Zona pellucida protein B; Processed zona pellucida sperm-binding protein 4) (Biotin); Processed zona pellucida sperm-binding protein 4; anti-ZP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ZP4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-482 amino acids from the C-terminal region of human ZP4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).)

Immunohistochemistry (IHC)

(ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ZP4 antibody

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.

Product Categories/Family for anti-ZP4 antibody

Antibodies; Binding Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): McCauley, J.L., et al. Genes Immun. 10(7):624-630(2009). Nakano, M., et al. Proc. Natl. Acad. Sci. U.S.A. 106(31):12838-12842(2009). Choudhury, S., et al. J. Reprod. Immunol. 79(2):137-147(2009). Chiu, P.C., et al. Biol. Reprod. 79(5):869-877(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 10863987
• NCBI GeneID: 57829
• NCBI Accession #: NP_067009.1
• NCBI GenBank Nucleotide #: NM_021186.3
• UniProt Accession #: Q12836; B2RAE1
• Molecular Weight: 59,400 Da
• NCBI Official Full Name: zona pellucida sperm-binding protein 4 preproprotein
• NCBI Official Synonym Full Names: zona pellucida glycoprotein 4
• NCBI Official Symbol: ZP4
• NCBI Official Synonym Symbols: ZBP; ZP1; ZPB; Zp-4
• NCBI Protein Information: zona pellucida sperm-binding protein 4; zona pellucida B protein; zona pellucida protein B
• UniProt Protein Name: Zona pellucida sperm-binding protein 4
• Protein Family: Zona pellucida sperm-binding protein
• UniProt Gene Name: ZP4
• UniProt Synonym Gene Names: ZPB; Zp-4
• UniProt Entry Name: ZP4_HUMAN

NCBI Description

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]

Uniprot Description

ZP4: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor. Belongs to the ZP domain family. ZPB subfamily.

Protein type: Membrane protein, integral; Extracellular matrix

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: proteinaceous extracellular matrix; plasma membrane; extracellular region; integral to membrane

Molecular Function: acrosin binding; signal transducer activity

Biological Process: positive regulation of humoral immune response; binding of sperm to zona pellucida; positive regulation of protein kinase activity; single fertilization; positive regulation of T cell proliferation; multicellular organism reproduction