Rabbit anti-Human LHX8 Polyclonal Antibody | anti-LHX8 antibody

LHX8, ID (LHX8, LIM/homeobox protein Lhx8) (APC)

Cat. Num. AAA6316010

• Gene Names: LHX8; LHX7
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LHX8; Polyclonal Antibody; ID (LHX8; LIM/homeobox protein Lhx8) (APC); LIM/homeobox protein Lhx8; anti-LHX8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-LHX8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LHX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the Central region of human LHX8.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LHX8 Antibody (Center) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the LHX8 antibody detected the LHX8 protein (arrow).)

Related Product Information for anti-LHX8 antibody

Members of the LIM homeobox gene family, such as LHX8, encode transcription regulators that share common structural features. They all contain 2 tandemly repeated cysteine-rich double-zinc finger motifs, called LIM domains, in addition to a homeodomain. The homeodomain is a DNA-binding domain, and the LIM domains are essential for regulating the activity of these molecules by interacting with other proteins. Members of the LIM homeobox gene family are required for the patterning or the specification and differentiation of different cell types during embryonic development (Zhao et al., 1999 [PubMed 10611327]).

Product Categories/Family for anti-LHX8 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756(2010). Jagomagi, T., et al. Eur. J. Oral Sci. 118(3):213-220(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008). Qin, Y., et al. Fertil. Steril. 89(4):1012-1014(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 50083287
• NCBI GeneID: 431707
• NCBI Accession #: NP_001001933.1
• NCBI GenBank Nucleotide #: NM_001001933.1
• UniProt Accession #: Q68G74; E9PGE3
• Molecular Weight: 38,170 Da
• NCBI Official Full Name: LIM/homeobox protein Lhx8 isoform 1
• NCBI Official Synonym Full Names: LIM homeobox 8
• NCBI Official Symbol: LHX8
• NCBI Official Synonym Symbols: LHX7
• NCBI Protein Information: LIM/homeobox protein Lhx8; LIM-homeodomain protein Lhx8
• UniProt Protein Name: LIM/homeobox protein Lhx8
• Protein Family: LIM/homeobox protein
• UniProt Gene Name: LHX8
• UniProt Synonym Gene Names: LIM homeobox protein 8
• UniProt Entry Name: LHX8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the LIM homeobox family of proteins, which are involved in patterning and differentiation of various tissue types. These proteins contain two tandemly repeated cysteine-rich double-zinc finger motifs known as LIM domains, in addition to a DNA-binding homeodomain. This family member is a transcription factor that plays a role in tooth morphogenesis. It is also involved in oogenesis and in neuronal differentiation. This gene is a candidate gene for cleft palate, and it is also associated with odontoma formation. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

LHX8: Transcription factor involved in differentiation of certain neurons and mesenchymal cells.

Protein type: DNA-binding; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: female germ cell nucleus

Molecular Function: zinc ion binding; sequence-specific DNA binding

Biological Process: learning and/or memory; regulation of transcription, DNA-dependent; transcription, DNA-dependent; forebrain neuron development; female gonad development; odontogenesis of dentine-containing teeth