Rabbit ZO-3 Polyclonal Antibody | anti-ZO-3 antibody
ZO-3, CT (Tight Junction-associated Polypeptide, Zonula Occludens 3) (APC)
Reactivity
Human, Mouse, Rat, Canine
Applications
Immunofluorescence, ELISA, Western Blot, Immunoprecipitation
Purity
Purified by Immunoaffinity chromatography.
Synonyms
ZO-3; Polyclonal Antibody; CT (Tight Junction-associated Polypeptide; Zonula Occludens 3) (APC); anti-ZO-3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Canine
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the human ZO-3 protein. MW: ~130kD. Crossreactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84, HeLa and canine MDCK cell lysates as well as mouse liver homogenates. Species Crossreactivity: canine, mouse and rat
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ZO-3 antibody
Immunoflourescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the C-terminus of the human ZO-3 protein.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Related Product Information for anti-ZO-3 antibody
ZO-3, a ~130kD protein, contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a SH3 domain, and a guanylate kinase-liked domain. Like ZO-1 and ZO-2, ZO-3 contains a C-terminal acidic domain and a basic region (between the first and second PDZ domains), making it belongs to the MAGUK protein family. By Western Blot analysis, ZO-3 is identified as a ~130kD protein that directly interacts with tight junction proteins ZO-1 and occuldin, but not with ZO-2. However, in one study, ZO-2, ZO-3 and Occludin colocalized with actin aggregates at cell border in MDCK cells. ZO-3 is localized to sites of cell-cell interaction, identical to that of ZO-1 in tight junctions. By immunoprecipitation analysis, ZO-3 co-immunoprecipitates with ZO-1. Using MDCK cells, ZO-3 protein colocalized with ZO-1 by immunofluorescence. In vitro binding assays, the N-terminal of ZO-3 showed preferably binding of ZO-1 and actin filaments (3). Moreover, the binding of ZO-1/ZO-2/ZO-3 was also found to interact with certain Claudin proteins.
Product Categories/Family for anti-ZO-3 antibody
References
1. Haskins, J., et al., J. Cell Biol. 141(1): 199-208 (1998). 2. Wittchen, E.S., et al., J. Biol. Chem. 274(49): 35, 179-35, 185 (1999). 3. Wittchen, E.S., et al., J. Cell Biol. 151(4): 825-836 (2000). 4. Itoh, M., et al., J. Cell Biol. 147(6): 1351-1363 (1999).
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Product Notes
The ZO-3 (Catalog #AAA6506654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZO-3, CT (Tight Junction-associated Polypeptide, Zonula Occludens 3) (APC) reacts with Human, Mouse, Rat, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's ZO-3 can be used in a range of immunoassay formats including, but not limited to, Immunoflourescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZO-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZO-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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