Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using ZNF387 antibody.)
Rabbit anti-Human ZNF387 Polyclonal Antibody | anti-ZNF387 antibody
ZNF387 Antibody
Gene Names
ST18; NZF3; ZNF387; ZC2H2C3; ZC2HC10
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ZNF387; Polyclonal Antibody; ZNF387 Antibody; KIAA0535; St18; ST18_HUMAN; suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein); Suppression of tumorigenicity 18 protein; Suppression of tumorigenicity protein 18; Zinc finger protein 387; Znf387; anti-ZNF387 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ZNF387 antibody detects endogenous levels of total ZNF387
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1047
Applicable Applications for anti-ZNF387 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using ZNF387 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using ZNF387 antibody.)
Immunofluorescene (IF)
(MBS9604492 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604492 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-ZNF387 antibody
Function: Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.
Similarity: Belongs to the MYT1 family.
Similarity: Belongs to the MYT1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 115 kD
Predicted: 116 kDa
Predicted: 116 kDa
NCBI Official Full Name
suppression of tumorigenicity 18 protein isoform a
NCBI Official Synonym Full Names
ST18, C2H2C-type zinc finger
NCBI Official Symbol
ST18
NCBI Official Synonym Symbols
NZF3; ZNF387; ZC2H2C3; ZC2HC10
NCBI Protein Information
suppression of tumorigenicity 18 protein
UniProt Protein Name
Suppression of tumorigenicity 18 protein
UniProt Gene Name
ST18
UniProt Synonym Gene Names
KIAA0535; ZNF387
Uniprot Description
Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (). Inhibits colony formation in cultured breast cancer cells.
Research Articles on ZNF387
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Product Notes
The ZNF387 st18 (Catalog #AAA9604492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZNF387 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF387 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ZNF387 st18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF387, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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