Rabbit anti-Human ZNF384 Polyclonal Antibody | anti-ZNF384 antibody

ZNF384, CT (ZNF384, CAGH1, CIZ, NMP4, TNRC1, Zinc finger protein 384, CAG repeat protein 1, CAS-interacting zinc finger protein, Nuclear matrix transcription factor 4, Trinucleotide repeat-containing gene 1 protein) (APC)

Cat. Num. AAA6366416

• Gene Names: ZNF384; NP; CIZ; NMP4; CAGH1; ERDA2; TNRC1; CAGH1A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZNF384; Polyclonal Antibody; CT (ZNF384; CAGH1; CIZ; NMP4; TNRC1; Zinc finger protein 384; CAG repeat protein 1; CAS-interacting zinc finger protein; Nuclear matrix transcription factor 4; Trinucleotide repeat-containing gene 1 protein) (APC); Trinucleotide repeat-containing gene 1 protein; anti-ZNF384 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 516

• Applicable Applications for anti-ZNF384 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZNF384 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-567 amino acids from the C-terminal region of human ZNF384.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ZNF384 Antibody (C-term) western blot analysis in MCF-7,Hela cell line lysates (35ug/lane).This demonstrates the ZNF384 antibody detected the ZNF384 protein (arrow).)

Flow Cytometry (FC/FACS)

(ZNF384 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ZNF384 antibody

This gene contains long CAG trinucleotide repeats coding consecutive glutamine residues. The gene product may functions as a transcription factor, with a potential role in the regulation of neurodevelopment or neuroplasticity. The protein appears to bind and regulate the promoters of MMP1, MMP3, MMP7 and COL1A1. Studies in mouse suggest that nuclear matrix transcription factors (NP/NMP4) may be part of a general mechanical pathway that couples cell construction and function during extracellular matrix remodeling. Multiple transcript variants encoding several isoforms have been found for this gene.

Product Categories/Family for anti-ZNF384 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Alves, J., et al. Biochem. Biophys. Res. Commun. 384(4):495-500(2009). Zhong, C.H., et al. Leukemia 22(4):723-729(2008). Janssen, H., et al. Exp. Cell Res. 312(7):1194-1204(2006). La Starza, R., et al. Leukemia 19(9):1696-1699(2005). Martini, A., et al. Cancer Res. 62(19):5408-5412(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 36054194
• NCBI GeneID: 171017
• NCBI Accession #: NP_597733.2
• NCBI GenBank Nucleotide #: NP_597733.2
• UniProt Accession #: Q8TF68
• NCBI Official Full Name: zinc finger protein 384 isoform a
• NCBI Official Synonym Full Names: zinc finger protein 384
• NCBI Official Symbol: ZNF384
• NCBI Official Synonym Symbols: NP; CIZ; NMP4; CAGH1; ERDA2; TNRC1; CAGH1A
• NCBI Protein Information: zinc finger protein 384
• UniProt Protein Name: Zinc finger protein 384
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF384
• UniProt Synonym Gene Names: CAGH1; CIZ; NMP4; TNRC1
• UniProt Entry Name: ZN384_HUMAN

NCBI Description

This gene encodes a C2H2-type zinc finger protein, which may function as a transcription factor. This gene also contains long CAG trinucleotide repeats that encode consecutive glutamine residues. The protein appears to bind and regulate the promoters of the extracellular matrix genes MMP1, MMP3, MMP7 and COL1A1. Studies in mouse suggest that nuclear matrix transcription factors (NP/NMP4) may be part of a general mechanical pathway that couples cell construction and function during extracellular matrix remodeling. Alternative splicing results in multiple transcript variants. Recurrent rearrangements of this gene with the Ewing's sarcoma gene, EWSR1 on chromosome 22, or with the TAF15 gene on chromosome 17, or with the TCF3 (E2A) gene on chromosome 19, have been observed in acute leukemia. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Apr 2011]

Uniprot Description

ZNF384: Transcription factor that binds the consensus DNA sequence [GC]AAAAA. Seems to bind and regulate the promoters of MMP1, MMP3, MMP7 and COL1A1. Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: focal adhesion; nucleus

Molecular Function: sequence-specific DNA binding; metal ion binding; SH3 domain binding

Biological Process: transcription, DNA-dependent; positive regulation of protein secretion; nucleocytoplasmic transport; positive regulation of transcription from RNA polymerase II promoter