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Western Blot (WB) (Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-ZIP7 (Ser275/Ser276) Antibody. The lane on the left was treated with blocking peptide.)

Rabbit ZIP7 Polyclonal Antibody | anti-ZIP7 antibody

Phospho-ZIP7 (Ser275/Ser276) Antibody

Gene Names
SLC39A7; KE4; HKE4; ZIP7; RING5; H2-KE4; D6S115E; D6S2244E
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
ZIP7; Polyclonal Antibody; Phospho-ZIP7 (Ser275/Ser276) Antibody; D6S115E; D6S2244E; H2 KE4; Histidine rich membrane protein Ke4; Histidine-rich membrane protein Ke4; HKE4; HLA class II region expressed gene KE4; KE4; Ke4 gene mouse; human homolog of; Really interesting new gene 5 protein; RING5; S39A7_HUMAN; SLC39A7; Solute carrier family 39 (zinc transporter); member 7; Solute carrier family 39 member 7; solute carrier family 39 zinc transporter member 7; Zinc transporter SLC39A7; anti-ZIP7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-ZIP7 (Ser275/Ser276) Antibody detects endogenous levels of ZIP7 only when phosphorylated at Ser275/276.
Tissue Specificity: Widely expressed.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ZIP7 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human ZIP7 around the phosphorylation site of Ser275/276.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Rapidly phosphorylated by CK2 following Zn (2+) treatment. This phosphorylation is required for efficient cytosolic Zn (2+) release.
Similarity
Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily.
Subcellular Location
Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus>cis-Golgi network membrane.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-ZIP7 (Ser275/Ser276) Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-ZIP7 (Ser275/Ser276) Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)

(Staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (Staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-ZIP7 antibody
Zinc transporter, that transports Zn (2+) from the endoplasmic reticulum/Golgi apparatus to the cytosol. Transport is stimulated by growth factors, such as EGF, and Ca (2+), as well as by exogenous Zn (2+).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,118 Da
NCBI Official Full Name
zinc transporter SLC39A7
NCBI Official Synonym Full Names
solute carrier family 39 (zinc transporter), member 7
NCBI Official Symbol
SLC39A7
NCBI Official Synonym Symbols
KE4; HKE4; ZIP7; RING5; H2-KE4; D6S115E; D6S2244E
NCBI Protein Information
zinc transporter SLC39A7; Ke4 gene, mouse, human homolog of; solute carrier family 39 member 7; histidine-rich membrane protein Ke4; really interesting new gene 5 protein; HLA class II region expressed gene KE4
UniProt Protein Name
Zinc transporter SLC39A7
UniProt Gene Name
SLC39A7
UniProt Synonym Gene Names
HKE4; RING5
UniProt Entry Name
S39A7_HUMAN

NCBI Description

The protein encoded by this gene transports zinc from the Golgi and endoplasmic reticulum to the cytoplasm. This transport may be important for activation of tyrosine kinases, some of which could be involved in cancer progression. Therefore, modulation of the encoded protein could be useful as a therapeutic agent against cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

SLC39A7: transports zinc from the Golgi and endoplasmic reticulum to the cytoplasm. This transport may be important for activation of tyrosine kinases, some of which could be involved in cancer progression. Therefore, modulation of the encoded protein could be useful as a therapeutic agent against cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: metal ion transmembrane transporter activity; protein binding

Biological Process: zinc ion transport; transmembrane transport

Research Articles on ZIP7

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Product Notes

The ZIP7 slc39a7 (Catalog #AAA9612934) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ZIP7 (Ser275/Ser276) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZIP7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). IHC: 1:50-1:200 WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the ZIP7 slc39a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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