Western Blot (WB)
(Western blot analysis of ZIP14 in human spleen tissue lysate with ZIP14 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit ZIP14 Polyclonal Antibody | anti-Slc39a14 antibody
ZIP14 Antibody
Gene Names
SLC39A14; NET34; ZIP14; cig19; LZT-Hs4
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
ZIP14 Antibody is affinity chromatography purified via peptide column.
Synonyms
ZIP14; Polyclonal Antibody; ZIP14 Antibody; NET34; cig19; LZT-Hs4; KIAA0062; Zinc transporter ZIP14; LIV-1 subfamily of ZIP zinc transporter 4; solute carrier family 39 (zinc transporter); member 14; anti-Slc39a14 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three isoforms of ZIP14 are known to exist; this antibody will detect both isoforms. ZIP14 antibody is predicted to not cross-react with other ZIP family members.
Purity/Purification
ZIP14 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; ZIP14 Antibody is supplied in PBS containing 0.02% sodium azide
Concentration
1 mg/mL (varies by lot)
Sequence Length
492
Applicable Applications for anti-Slc39a14 antibody
ELISA. IF, IHC-P, WB
Application Notes
ZIP11 antibody can be used for detection of ZIP11 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Optimal dilutions for each application to be determined by the researcher.
Optimal dilutions for each application to be determined by the researcher.
Immunogen
ZIP14 antibody was raised against a 16 amino acid synthetic peptide near the center of human ZIP14.
Immunogen is located withing amino acids 230-280 of ZIP14
Immunogen is located withing amino acids 230-280 of ZIP14
Positive Control
MBS151674-Human Spleen Tissue Lysate.
Homology
Predicted species reactivity bases on immunogen sequence: Bovine: (94%).
Preparation and Storage
ZIP14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ZIP14 in human spleen tissue lysate with ZIP14 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of ZIP14 in human spleen tissue lysate with ZIP14 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ZIP14 in mouse liver tissue with ZIP14 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of ZIP14 in mouse liver tissue with ZIP14 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZIP14 in Mouse Liver tissue with ZIP14 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ZIP14 in Mouse Liver tissue with ZIP14 antibody at 20 μg/mL.)
Related Product Information for anti-Slc39a14 antibody
ZIP14 Antibody: The zinc transporter ZIP14, also known as SLC39A14, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP14 is a glycosylated multipass plasma membrane protein that belongs to the ZIP transporter subfamily LIV-1. ZIP14 has been shown to contribute to the hypozincemia of inflammation and infection and is regulated in the liver by IL-6. In addition to zinc, ZIP14 is also involved in the cellular uptake of non-transferrin-bound iron as well as iron bound to transferrin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 54kDa
Observed MW: 52kDa
Observed MW: 52kDa
NCBI Official Full Name
zinc transporter ZIP14 isoform a
NCBI Official Synonym Full Names
solute carrier family 39 (zinc transporter), member 14
NCBI Official Symbol
SLC39A14
NCBI Official Synonym Symbols
NET34; ZIP14; cig19; LZT-Hs4
NCBI Protein Information
zinc transporter ZIP14; ZIP-14; Zrt-, Irt-like protein 14; zrt- and Irt-like protein 14; solute carrier family 39 member 14; LIV-1 subfamily of ZIP zinc transporter 4; solute carrier family 39 (metal ion transporter), member 14
UniProt Protein Name
Zinc transporter ZIP14
Protein Family
UniProt Gene Name
SLC39A14
UniProt Synonym Gene Names
KIAA0062; ZIP14; LZT-Hs4; ZIP-14
UniProt Entry Name
S39AE_HUMAN
NCBI Description
Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A14 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson, 2003 [PubMed 12659941]).[supplied by OMIM, Mar 2008]
Uniprot Description
SLC39A14: May mediate cellular uptake of nontransferrin-bound iron. Broad-scope metal ion transporter with a preference for zinc uptake. Belongs to the ZIP transporter (TC 2.A.5) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter
Chromosomal Location of Human Ortholog: 8p21.3
Cellular Component: Golgi apparatus; lamellipodium; integral to plasma membrane; endoplasmic reticulum; integral to membrane; plasma membrane
Molecular Function: zinc ion transmembrane transporter activity; ferrous iron transmembrane transporter activity
Biological Process: ferrous iron transport; cellular zinc ion homeostasis; transmembrane transport
Research Articles on Slc39a14
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Product Notes
The Slc39a14 slc39a14 (Catalog #AAA151580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZIP14 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZIP14 can be used in a range of immunoassay formats including, but not limited to, ELISA. IF, IHC-P, WB. ZIP11 antibody can be used for detection of ZIP11 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the Slc39a14 slc39a14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIP14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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